Invitrogen

VGLL4 Recombinant Rabbit Monoclonal Antibody (16H37L22)

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

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抗体检测数据 (3)
高级验证 (1)

VGLL4 Antibody in Immunocytochemistry (ICC/IF)

VGLL4 Antibody in Immunocytochemistry (ICC/IF)

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VGLL4 Antibody (703699) in ICC/IF

For immunofluorescence analysis, HCT 116 cells were fixed and permeabilized for detection of endogenous VGLL4 using Anti-VGLL4 Recombinant Rabbit Monoclonal Antibody (Product # 703699, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of VGLL4 protein (green), Panel b) is stained for nuclei (blue) using ProLong™ Diamond Antifade Mountant with DAPI (Pro... View More

VGLL4 Antibody in Immunocytochemistry (ICC/IF)

VGLL4 Antibody in Western Blot (WB)

VGLL4 Antibody in Western Blot (WB)

Product Image Gallery

VGLL4 Antibody in Immunocytochemistry (ICC/IF) — For immunofluorescence analysis, HCT 116 cells were fixed and permeabilized for detection of endogenous VGLL4 using Anti-VGLL4 Recombinant Rabbit Monoclonal Antibody (Product # 703699, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of VGLL4 protein (green), Panel b) is stained for nuclei (blue) using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of VGLL4. Panel e) represents control cells with no primary antibody to assess the background. The images were captured at 60X magnification.

VGLL4 Antibody in Western Blot (WB) — Western blot was performed using Anti-VGLL4 Recombinant Rabbit Monoclonal Antibody (Product # 703699) and a 34 kDa band corresponding to VGLL4 was observed across the cell line tested. Modified whole cell extracts (1% SDS) (30 µg lysate) of HCT 116 (Lane 1), Caco-2 (Lane 2), U87-MG (Lane3), SH-SY5Y (Lane4) and HeLa (Lane 5) were electrophoresed using Novex® NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2500 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:5000 dilution). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

VGLL4 Antibody in Western Blot (WB) — Knockdown of VGLL4 was achieved by transfecting HCT 116 cells with VGLL4 specific siRNA (Silencer® select Product # s229764 & s229766). Western blot analysis (Fig a) was performed using modified whole cell extracts (1% SDS) from VGLL4 knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with Anti-VGLL4 Recombinant Rabbit Monoclonal Antibody (Product # 703699, 1:2500 dilution) and Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:5000 dilution). Densitometric analysis of this western blot is shown in the histogram (Fig b). Loss of signal upon siRNA mediated knockdown confirms that the antibody is specific to VGLL4.

VGLL4 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of the target protein. HCT 116 cells were transfected with VGLL4 siRNA and decrease in signal intensity was observed in western blot application using Anti-VGLL4 Recombinant Rabbit Monoclonal Antibody (Product # 703699). {KD}

产品信息

703699

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,500

-

免疫细胞化学 (ICC/IF)

1:100

-

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

16H37L22

抗原

Peptides corresponding to human VGLL4 [1) aa6-aa23 2) aa235-aa253]

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2866468

产品详细信息

This antibody is predicted to react with Mouse, Rat, Bovine.

靶标信息

Transcription cofactor vestigial-like protein 4 (VGLL4) acts as a specific coactivator for the mammalian TEFs.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Transcription cofactor vestigial-like protein 4; vestigial like 4; Vestigial-like 4; vestigial-like family member 4; Vgl-4

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基因别名: KIAA0121; VGL-4; VGLL4

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UniProt ID: (Human) Q14135

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Entrez Gene ID: (Human) 9686

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protein binding transcription cofactor

参与通路

transcription, DNA-templated regulation of transcription, DNA-templated