Invitrogen

VIP Polyclonal Antibody

VIP Antibody in Immunocytochemistry (ICC/IF)

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VIP Antibody (PA5-78224) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of VIP was performed in DIV9 rat E18 primary hippocampal neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: VIP Polyclonal Antibody (Product # PA5-78224) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.

VIP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

VIP Antibody in Immunohistochemistry (Frozen) (IHC (F))

VIP Antibody in Immunohistochemistry (IHC)

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产品信息

PA5-78224

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

免疫组化 (IHC)

查看6篇已发表文章 6篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫组化（冰冻） (IHC (F))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human VIP. The exact sequence is proprietary.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.16 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol, 1% BSA

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2736784

产品详细信息

Positive Control: SK-N-SH , SK-N-AS, PC-3

Predicted Reactivity: Pig (100%), Sheep (100%), Rhesus Monkey (100%), Bovine (100%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

VIP encodes a protein that belongs to the glucagon family. It stimulates myocardial contractility, causes vasodilation, increases glycogenolysis, lowers arterial blood pressure and relaxes the smooth muscle of trachea, stomach and gall bladder. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Tiered sympathetic control of cardiac function revealed by viral tracing and single cell transcriptome profiling.

eLife

Sharma S,Littman R,Tompkins JD,Arneson D,Contreras J,Dajani AH,Ang K,Tsanhani A,Sun X,Jay PY,Herzog H,Yang X,Ajijola OA

PA5-78224 was used in Immunohistochemistry to target specific neuronal subtypes for sympathetic blockade in cardiac disease.

Wed May 10 00:00:00 EDT 2023

Activation of the anterior cingulate cortex ameliorates anxiety in a preclinical model of fetal alcohol spectrum disorders.

Translational psychiatry

Hwang HM,Hashimoto-Torii K

Published figure using VIP polyclonal antibody (Product # PA5-78224) in Immunohistochemistry

Thu Jan 20 00:00:00 EST 2022

Activation of the anterior cingulate cortex ameliorates anxiety in a preclinical model of fetal alcohol spectrum disorders.

Translational psychiatry

Hwang HM,Hashimoto-Torii K

Published figure using VIP polyclonal antibody (Product # PA5-78224) in Immunohistochemistry

Thu Jan 20 00:00:00 EST 2022

Identification of TPBG-Expressing Amacrine Cells in DAT-tdTomato Mouse.

Investigative ophthalmology & visual science

Huang W,Xu Q,Liu F,Su J,Xiao D,Tang L,Hao ZZ,Liu R,Xiang K,Bi Y,Miao Z,Liu X,Liu Y,Liu S

PA5-78224 was used in Immunohistochemistry to identify trophoblast glycoprotein (TPBG)-expressing GABAergic amacrine cells (ACs) that were selectively labeled in DAT-tdTomato transgenic mice.

Mon May 02 00:00:00 EDT 2022

Loss of ARHGAP15 affects the directional control of migrating interneurons in the embryonic cortex and increases susceptibility to epilepsy.

Frontiers in cell and developmental biology

Liaci C,Camera M,Zamboni V,Sarò G,Ammoni A,Parmigiani E,Ponzoni L,Hidisoglu E,Chiantia G,Marcantoni A,Giustetto M,Tomagra G,Carabelli V,Torelli F,Sala M,Yanagawa Y,Obata K,Hirsch E,Merlo GR

PA5-78224 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to indicate that ARHGAP15 imposes a fine negative regulation on RAC1 that is required for morphological maturation and directional control during CIN migration, with consequences on their laminar distribution and inhibitory function.