Invitrogen

VPS34 Monoclonal Antibody (H.107.3)

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (2)
高级验证 (2)

VPS34 Antibody in Immunocytochemistry (ICC/IF)

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VPS34 Antibody (MA5-14791) in ICC/IF

Immunofluorescence analysis of Phosphatidylinositol 3-kinase catalytic subunit type 3 was performed using 70% confluent log phase A-375 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with VPS34 Monoclonal Antibody (H.107.3) (Product # MA5-14791) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary... View More

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VPS34 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across Reh, A375, HT1080, U2OS, HT-29, PC3, Hep G2. owing to their inherent genetic constitution. Relative expression of Phosphatidylinositol 3-kinase catalytic subunit type 3 was observed in Reh, A375, HT1080, HT-29 and PC3 but not in U2OS and Hep G2 using Anti-VPS34 Monoclonal Antibody (H.107.3) (Product # MA5-14791) in Western Blot. {RE}

产品信息

MA5-14791

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human, Mouse, Non-human primate, Rat

宿主/亚型

Rabbit / IgG

分类

Monoclonal

类型

Antibody

克隆号

H.107.3

抗原

Synthetic peptide corresponding to residues in the amino-terminal sequence of human PI3K class III

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

95 µg/mL

纯化类型

Affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

<0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_10975346

产品详细信息

It is not recommended to aliquot this antibody.

靶标信息

PIK3C3 (hVPS34) is the sole class III member of the PI3 Kinase family. PIK3C3 phosphorylates phosphatidylinositol (PI) to produce phosphatidylinositol 3-phosphate [PI(3)P], which recruits downstream effectors containing FYVE and Phox domains to membranes. PIK3C3 is involved in coupling nutrient signaling to mTOR and also plays a role in autophagy and intracellular trafficking. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for transport from early to late endosomes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: catalytic phosphatidylinositol 3-kinase 3; catalytic subunit type 3 Antibody; Class III PI 3-kinase; hVps34; hVps34 Antibody; OTTMUSP00000023341; OTTMUSP00000023350; phosphatidylinositol 3-kinase; Phosphatidylinositol 3-kinase catalytic subunit type 3; Phosphatidylinositol 3-kinase p100 subunit; phosphatidylinositol 3-kinase, catalytic subunit type 3; Phosphoinositide-3-kinase class 3; phosphoinositide-3-kinase, class 3; PI-3K Vps34p; PI3-kinase type 3; PI3K type 3; pi3kc3; PIK3C3 Antibody; PtdIns-3-kinase type 3

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基因别名: 5330434F23Rik; hVps34; PIK3C3; VPS34

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UniProt ID: (Human) Q8NEB9, (Rat) O88763, (Mouse) Q6PF93

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Entrez Gene ID: (Human) 5289, (Rat) 65052, (Mouse) 225326

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