Invitrogen

VPS35 Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1 篇参考文献

查看其他28个VPS35抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (11)
高级验证 (2)

VPS35 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 13

VPS35 Antibody (PA5-21898) in ICC/IF

Immunofluorescence analysis of VPS35 was performed using U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with VPS35 Rabbit Polyclonal Antibody (Product # PA5-21898) at 1:200 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of... View More

VPS35 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

VPS35 Antibody in Immunoprecipitation (IP)

Product Image Gallery

VPS35 Antibody — Antibody specificity was demonstrated by siRNA mediated KD of target protein. HeLa cells were transfected with VPS35 siRNA and reduction of signal was observed in Western Blot using VPS35 Polyclonal Antibody (Product # PA5-21898). {KD}

产品信息

PA5-21898

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:10,000

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

免疫沉淀 (IP)

Assay-dependent

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to a region within amino acids 733 and 796 of VPS35 (Uniprot ID#Q96QK1)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.66 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_11153540

产品详细信息

Recommended positive controls: Mouse brain, Rat Brain, Hela, A549, H1299, HCT116, HepG2, Molt4, Raji.

Predicted reactivity: Mouse (100%), Rat (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Dopamine Transporter Localization in Medial Forebrain Bundle Axons Indicates Its Long-Range Transport Primarily by Membrane Diffusion with a Limited Contribution of Vesicular Traffic on Retromer-Positive Compartments.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Bagalkot TR,Block ER,Bucchin K,Balcita-Pedicino JJ,Calderon M,Sesack SR,Sorkin A

PA5-21898 was used in Immunohistochemistry to suggest that DAT traffics between midbrain and striatum mainly by lateral diffusion in the plasma membrane with only a limited contribution of vesicular transport in recycling endosomes.

Wed Jan 13 00:00:00 EST 2021

生物信息学

蛋白别名: DKFZp434E1211; DKFZp434P1672; FLJ10752; FLJ13588; FLJ20388; hVPS35; Maternal embryonic message 3; Maternal-embryonic 3; vacuolar protein sorting 35 homolog; Vacuolar protein sorting-associated protein 35; Vesicle protein sorting 35

查看更多收起

基因别名: AI647796; MEM3; PARK17; TCCCTA00141; VPS35

查看更多收起

UniProt ID: (Human) Q96QK1, (Mouse) Q9EQH3

查看更多收起

Entrez Gene ID: (Human) 55737, (Rat) 25479, (Mouse) 65114

查看更多收起

功能

protein binding protein transporter activity membrane traffic protein

参与通路