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VPS35 Antibody (MA5-45936) in WB
Western Blot analysis of Human, Mouse A549, MEF showing detection of VPS35 protein. Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells. Load: 8 µg each A549 and MEF. Samples were incubated with VPS35 monoclonal antibody (Product # MA5-45936) at 1:5 (tissue culture supernatant), followed by Donkey anti-mouse IRDye 800CW at 1:25,000 in TBS-T.
产品信息
MA5-45936
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
7E4
抗原
Full length recombinant human VSP35
偶联物
激发/发射光谱
651/660 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
47.35mM phosphate/2.15mM MES, pH 7.4, with 2mM EDTA, 0.17066µM Pentachlorophenol, 0.8mM ethanol
内含物
no preservative
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2932390
产品详细信息
A 1:1,000 dilution of MA5-45936 was sufficient for detection of VPS35 in 10 µg of SH-SY5Y by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.|Detects approximately 92 kDa.
靶标信息
This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DKFZp434E1211; DKFZp434P1672; FLJ10752; FLJ13588; FLJ20388; hVPS35; Maternal embryonic message 3; Maternal-embryonic 3; vacuolar protein sorting 35 homolog; Vacuolar protein sorting-associated protein 35; Vesicle protein sorting 35
基因别名: AI647796; MEM3; PARK17; TCCCTA00141; VPS35
UniProt ID: (Human) Q96QK1, (Mouse) Q9EQH3
Entrez Gene ID: (Human) 55737, (Rat) 25479, (Mouse) 65114
intracellular protein transport
Wnt signaling pathway
regulation of macroautophagy
regulation of protein stability
retrograde transport, endosome to Golgi
transcytosis
positive regulation of Wnt protein secretion
positive regulation of canonical Wnt signaling pathway
negative regulation of late endosome to lysosome transport
retrograde transport, endosome to plasma membrane
vacuolar protein processing
transport
protein transport
