Loading ...

Invitrogen

VWF Polyclonal Antibody

Advanced Verification

4 Published Figures

13 References

View all (49) VWF antibodies

Datasheet

Protocols

Tech Support

Catalog # PA5-16634

This product has been discontinued

500 µL

Datasheet

Protocols

Tech Support

Application

VWF Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 9

VWF Antibody (PA5-16634) in IHC (P)

Immunohistochemistry analysis of von Willebrand Factor showing secreted staining of paraffin-embedded human tonsil tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room tempera... View More

VWF Antibody in Immunocytochemistry (ICC/IF)

VWF Antibody in Immunohistochemistry (IHC)

VWF Antibody in Proximity Ligation Assay (PLA) (PLA)

Product Image Gallery

VWF Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of VWF was observed in all cell lines and tissues tested using VWF Polyclonal antibody (Product # PA5-16634) in western blot. {RE}

Product Details

PA5-16634

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:80-1:160

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

in situ PLA (PLA)

View 1 publication 1 publication

Product Specifications

Published species

Human, Mouse, Pig

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Purified factor VIII related antigen/von Willebrand factor

Conjugate

Unconjugated

Form

Liquid

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10982615

Product Specific Information

PA5-16634 targets von Willebrand Factor in IF and IHC (P) applications and shows reactivity with Human samples.

The PA5-16634 immunogen is purified factor VIII related antigen/von Willebrand factor.

Target Information

Von Willebrand factor (vWf) is a series of self-aggregated structures, all derived from common glycoprotein subunits, synthesized in endothelial cells and megakaryocytes. The molecular mass of the monomeric vWf unit is 225 kDa. vWf binds to circulating factor VIIIc (antihemophilic factor), hereby stabilizing the latter thus being an important factor in the normal primary haemostasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Robust critical limb ischemia porcine model involving skeletal muscle necrosis.

Scientific reports

El Masry MS,Gnyawali SC,Sen CK

PA5-16634 was used in Immunohistochemistry to successfully establish a translational model of severe hindlimb ischemia causing ischemic myopathy that enables long-term survival studies in compliance with regulatory requirements pertaining to animal welfare.

Tue Jul 18 00:00:00 UTC 2023

Non-invasive administration of AAV to target lung parenchymal cells and develop SARS-CoV-2-susceptible mice.

Molecular therapy : the journal of the American Society of Gene Therapy

Yang MS,Park MJ,Lee J,Oh B,Kang KW,Kim Y,Lee SM,Lim JO,Jung TY,Park JH,Park SC,Lim YS,Hwang SB,Lyoo KS,Kim DI,Kim B

PA5-16634 was used in Immunohistochemistry to demonstrate the ease and rapidness of the intratracheal administration of AAV for targeting lung tissue in mice, and this can be used to study diverse pulmonary diseases.

Wed May 04 00:00:00 UTC 2022

Reduced cleavage of von willebrand factor by ADAMTS13 is associated with microangiopathic acute kidney injury following trauma.

Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis

Plautz WE,Haldeman SH,Dyer MR,Sperry JL,Guyette FX,Loughran PA,Alvikas J,Hassoune A,Hoteit L,Alsaadi N,Zuckerbraun BS,Rollins-Raval MA,Raval JS,Mota RI,Neal MD

PA5-16634 was used in Immunohistochemistry (Paraffin), Western Blot to reveal that following traumatic injury vWF-ADAMTS13 axis shifts towards a prothrombotic state, and vWF-containing microangiopathic deposits concurrently produced potentially contribute to AKI development.

Sat Jan 01 00:00:00 UTC 2022

Infarct-preconditioning exosomes of umbilical cord mesenchymal stem cells promoted vascular remodeling and neurological recovery after stroke in rats.

Stem cell research & therapy

Ye YC,Chang ZH,Wang P,Wang YW,Liang J,Chen C,Wang JJ,Sun HT,Wang Y,Li XH

PA5-16634 was used in Immunocytochemistry-immunoflourescence to investigate if exosomes secreted in response to infarction microenvironment could have further therapeutic effects.

Thu Jul 28 00:00:00 UTC 2022

Dual roles of p62/SQSTM1 in the injury and recovery phases of acetaminophen-induced liver injury in mice.

Acta pharmaceutica Sinica. B

Qian H,Bai Q,Yang X,Akakpo JY,Ji L,Yang L,Rülicke T,Zatloukal K,Jaeschke H,Ni HM,Ding WX

PA5-16634 was used in Western Blot, Immunohistochemistry to investigate the role of p62/SQSTM1 (referred to as p62) in APAP-induced liver injury (AILI) in mice.

Wed Dec 01 00:00:00 UTC 2021

Dual roles of p62/SQSTM1 in the injury and recovery phases of acetaminophen-induced liver injury in mice.

Acta pharmaceutica Sinica. B

Qian H,Bai Q,Yang X,Akakpo JY,Ji L,Yang L,Rülicke T,Zatloukal K,Jaeschke H,Ni HM,Ding WX

PA5-16634 was used in Western Blot, Immunohistochemistry to investigate the role of p62/SQSTM1 (referred to as p62) in APAP-induced liver injury (AILI) in mice.

Wed Dec 01 00:00:00 UTC 2021

Myeloid lineage contributes to pathological choroidal neovascularization formation via SOCS3.

EBioMedicine

Wang T,Zhou P,Xie X,Tomita Y,Cho S,Tsirukis D,Lam E,Luo HR,Sun Y

PA5-16634 was used in Immunohistochemistry (PFA fixed), Immunohistochemistry to investigate the neovascular aspect of human age-related macular degeneration in relation to the suppressor of cytokine signaling 3 (SOCS3) pathway.

Mon Nov 01 00:00:00 UTC 2021

Myeloid lineage contributes to pathological choroidal neovascularization formation via SOCS3.

EBioMedicine

Wang T,Zhou P,Xie X,Tomita Y,Cho S,Tsirukis D,Lam E,Luo HR,Sun Y

Mon Nov 01 00:00:00 UTC 2021

Atrial natriuretic peptide promotes uterine decidualization and a TRAIL-dependent mechanism in spiral artery remodeling.

The Journal of clinical investigation

Zhang W,Li S,Lou J,Li H,Liu M,Dong N,Wu Q

PA5-16634 was used in Immunohistochemistry (Paraffin), Western Blot to indicate that ANP promotes decidualization and TRAIL expression in endometrial stromal cells, contributing to sequential events in remodeling of spiral arteries, including SMC death and cyclophilin B release, which in turn induces TRAIL receptor expression and apoptosis in ECs.

Fri Oct 15 00:00:00 UTC 2021

Increased talin-vinculin spatial proximities in livers in response to spotted fever group rickettsial and Ebola virus infections.

Laboratory investigation; a journal of technical methods and pathology

Liu Y,Xiao J,Zhang B,Shelite TR,Su Z,Chang Q,Judy B,Li X,Drelich A,Bei J,Zhou Y,Zheng J,Jin Y,Rossi SL,Tang SJ,Wakamiya M,Saito T,Ksiazek T,Kaphalia B,Gong B

Published figure using VWF polyclonal antibody (Product # PA5-16634) in Proximity Ligation Assay (PLA)

Sat Aug 01 00:00:00 UTC 2020

Increased talin-vinculin spatial proximities in livers in response to spotted fever group rickettsial and Ebola virus infections.

Laboratory investigation; a journal of technical methods and pathology

Liu Y,Xiao J,Zhang B,Shelite TR,Su Z,Chang Q,Judy B,Li X,Drelich A,Bei J,Zhou Y,Zheng J,Jin Y,Rossi SL,Tang SJ,Wakamiya M,Saito T,Ksiazek T,Kaphalia B,Gong B

Published figure using VWF polyclonal antibody (Product # PA5-16634) in Proximity Ligation Assay (PLA)

Sat Aug 01 00:00:00 UTC 2020

Identification of a pro-angiogenic functional role for FSP1-positive fibroblast subtype in wound healing.

Nature communications

Saraswati S,Marrow SMW,Watch LA,Young PP

Published figure using VWF polyclonal antibody (Product # PA5-16634) in Immunocytochemistry

Tue Jul 09 00:00:00 UTC 2019

Efficient One-Step Production of Microencapsulated Hepatocyte Spheroids with Enhanced Functions.

Small (Weinheim an der Bergstrasse, Germany)

Chan HF,Zhang Y,Leong KW

PA5-16634 was used in immunocytochemistry to analyze microencapsulated hepatocyte spheroids with enhanced functions and their efficient one-step production

Sun May 01 00:00:00 UTC 2016

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

VWF Polyclonal Antibody

VWF Antibody (PA5-16634) in IHC (P)

Product Details

PA5-16634

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

in situ PLA (PLA)

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Performance Guarantee

We're here to help

Your items have has been added!

VWF Polyclonal Antibody

VWF Antibody (PA5-16634) in IHC (P)

Product Details

PA5-16634

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

in situ PLA (PLA)

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Storage conditions

Shipping conditions

RRID .st0{fill-rule:evenodd;clip-rule:evenodd;fill:#A2A2A2;}

Product Specific Information

Target Information

Related Products for

Performance Guarantee

We're here to help

Your items have has been added!

RRID