Invitrogen

Vimentin Polyclonal Antibody

Vimentin Antibody in Immunocytochemistry (ICC/IF)

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Vimentin Antibody (PA5-142829) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of Vimentin in U2OS cells using Vimentin Polyclonal Antibody (Product # PA5-142829). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (5 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic/Intermediate filament staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Vimentin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Vimentin Antibody in Flow Cytometry (Flow)

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产品信息

PA5-142829

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-2 µg/mL

免疫组化（石蜡） (IHC (P))

5 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

流式细胞分析 (Flow)

10 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

Peptide with sequence C-QVINETSQHHDDLE.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Ammonium sulfate precipitation

保存液

TBS, pH 7.3, with 0.5% BSA

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2933472

产品详细信息

This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:4,000.

靶标信息

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。