Invitrogen

Vimentin Monoclonal Antibody (VI-01)

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

1 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (7)
高级验证 (2)

Vimentin Antibody in Immunocytochemistry (ICC/IF)

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Vimentin Antibody (MA1-19168) in ICC/IF

mmunofluorescence analysis of Vimentin was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Vimentin Mouse Monoclonal Antibody (Product # MA1-19168) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree Celsius and then labeled with Goat anti-Mouse IgG (H+L)/IgM (L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product #... View More

Vimentin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Vimentin Antibody in Flow Cytometry (Flow)

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产品信息

MA1-19168

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

免疫组化（石蜡） (IHC (P))

Assay-dependent

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

Assay-dependent

流式细胞分析 (Flow)

1-5 µg/mL

产品规格

种属反应

Human, Mammal, Mouse, Pig

已发表种属

Not Applicable

宿主/亚型

Mouse / IgM

分类

Monoclonal

类型

Antibody

克隆号

VI-01

抗原

Pellet of pig brain cold stable proteins after depolymerization of Microtubules.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

sequential chromatography

保存液

TBS, pH 8

内含物

15mM sodium azide

保存条件

4° C, do not freeze

运输条件

Wet ice

RRID

AB_1088258

产品详细信息

This antibody reacts with vimentin, a 57 kDa intermediate filament intracellular protein expressed in variety of mesenchymal and mesodermal cell types. Cross-reactivity was found with smooth muscle desmin.

Immunocytochemistry: Staining technique: (a) fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. Positive control: 3T3 murine Swiss albino fibroblast cell line, RBL rat basophilic leukemia cell line.

Flow cytometry: Intracellular staining.

靶标信息

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。