Invitrogen

WNT7A/WNT7B Polyclonal Antibody

WNT7A/WNT7B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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WNT7A/WNT7B Antibody (PA5-47430) in IHC (P)

Immunohistochemical analysis of WNT7A/WNT7B in immersion fixed paraffin-embedded sections of human endometrial cancer tissue. Samples were incubated in WNT7A/WNT7B polyclonal antibody (Product # PA5-47430) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).

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产品信息

PA5-47430

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1 µg/mL

免疫组化（石蜡） (IHC (P))

5-15 µg/mL

产品规格

种属反应

Human

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

E. coli-derived recombinant human Wnt-7a Leu32-Glu72, Thr216-Arg292

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.2 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5% trehalose

内含物

No Preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2610494

产品详细信息

In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse (rm) Wnt-1, rmWnt-3a, rmWnt-4, rmWnt-5a, rmWnt-5b, rmWnt-9a, rmWnt-11, and rmWnt-16 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

靶标信息

Wnt proteins constitute a family of secreted glycoproteins that activate signal transduction pathways to mediate tissue homeostasis, cell fate, cell proliferation, and self-renewal. The canonical signaling pathway is initiated upon Wnt binding to a Frizzled family receptor and an LRP5/LRP6 coreceptor, which leads to activation of the beta-catenin cascade. Non-canonical Wnt signals are activated by binding to a Frizzled family receptor and ROR2 coreceptor. Extensive research has shown that deregulation of these pathways or its components can lead to cancer. Human Wnt-1 is encoded as a 370 amino acid precursor that, upon secretion, is cleaved into a 343 amino acid (42 kDa) mature form. At the amino acid level, mature human Wnt-1 is approximately 99% identical to mature mouse Wnt-1. Depending on the tissue, Wnt-1 can activate the canonical and non-canonical signaling pathways. Studies have demonstrated its involvement in a variety of functions, including embryonic development and carcinogenesis. Wnt-1 signaling can also induce the phosphorylation and subsequent degradation of c-Myb.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Protein Wnt-7a; Protein Wnt-7b; proto-oncogene Wnt7a protein; wingless-type MMTV integration site family member 7A; wingless-type MMTV integration site family member 7B; wingless-type MMTV integration site family, member 7A; wingless-type MMTV integration site family, member 7B

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基因别名: WNT7A; WNT7B

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UniProt ID: (Human) O00755, (Human) P56706

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Entrez Gene ID: (Human) 7476, (Human) 7477

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功能

receptor binding frizzled binding cytokine activity protein binding receptor agonist activity intercellular signal molecule

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