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Application
FIGURE: 1 / 3
WNT7B Antibody (PA5-103480) in ICC/IF
Immunocytochemistry analysis of WNT7B in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with WNT7B polyclonal antibody (Product # PA5-103480) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-103480
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Not Applicable
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human WNT7B(Accession P56706), corresponding to amino acid residues N276-C326.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2852816
Product Specific Information
Antibody detects endogenous levels of total WNT7B.
Target Information
Wnt7b encodes a member of the WNT gene family, which consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Among members of the human WNT family, this gene product is most similar to WNT7A protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Protein Wnt-7b; wingless-related MMTV integration site 7B; wingless-type MMTV integration site family member 7B; wingless-type MMTV integration site family, member 7B
Gene Aliases: Wnt-7b; WNT7B
UniProt ID: (Human) P56706, (Mouse) P28047
Entrez Gene ID: (Human) 7477, (Rat) 315196, (Mouse) 22422
in utero embryonic development
metanephros morphogenesis
Wnt signaling pathway
establishment or maintenance of polarity of embryonic epithelium
forebrain regionalization
central nervous system vasculogenesis
neuron differentiation
lung development
oxygen homeostasis
homeostatic process
cellular metabolic process
cell fate commitment
positive regulation of osteoblast differentiation
fibroblast proliferation
embryonic organ development
synapse organization
canonical Wnt signaling pathway
lung morphogenesis
lung epithelium development
lobar bronchus development
trachea cartilage morphogenesis
developmental growth involved in morphogenesis
embryonic placenta morphogenesis
chorio-allantoic fusion
mammary gland epithelium development
lens fiber cell development
cellular response to retinoic acid
renal inner medulla development
renal outer medulla development
outer medullary collecting duct development
inner medullary collecting duct development
stem cell proliferation
metanephric collecting duct development
metanephric epithelium development
metanephric loop of Henle development
angiogenesis
vasculature development
activation of JUN kinase activity
positive regulation of cell proliferation
positive regulation of gene expression
cell proliferation in forebrain
neuron projection development
regulation of cell projection size
odontogenesis of dentin-containing tooth
negative regulation of smoothened signaling pathway
positive regulation of JNK cascade
neuron projection morphogenesis
stem cell development
negative regulation of neurogenesis
smooth muscle cell differentiation
response to glucocorticoid
anatomical structure regression
lung-associated mesenchyme development
apoptotic process involved in patterning of blood vessels
signal transduction
multicellular organism development
positive regulation of receptor activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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