Invitrogen

WT1 Monoclonal Antibody (6F-H2)

WT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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WT1 Antibody (MA1-46028) in IHC (P)

Immunohistochemical analysis of WT1 in human renal cancer. Samples were incubated with WT1 monoclonal antibody (Product # MA1-46028) followed by DAB with hematoxylin counterstain.

WT1 Antibody in Immunocytochemistry (ICC/IF)

WT1 Antibody in Immunohistochemistry (IHC)

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产品信息

MA1-46028

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2 µg/mL

免疫组化 (IHC)

查看5篇已发表文章 5篇已发表文章

免疫组化（石蜡） (IHC (P))

1:400

查看2篇已发表文章 2篇已发表文章

免疫组化（冰冻） (IHC (F))

1:400

免疫细胞化学 (ICC/IF)

Assay-Dependent

查看2篇已发表文章 2篇已发表文章

流式细胞分析 (Flow)

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

2-10 µg/mL lysate

产品规格

种属反应

Human

已发表种属

Human, Mouse, Rat

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

6F-H2

抗原

Recombinant protein corresponding to residues 1-181 of human WT1.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.0 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_962464

产品详细信息

MA1-46028 detects an epitope within residues 1-84.

靶标信息

The Wilms Tumor 1 gene encodes one of the WPP domain-interacting proteins (Wilms Tumor 1/AT5G11390, WIT2/AT1G68910) required for RanGAP nuclear envelope association in root tip cells. Ran GTPase plays essential roles in multiple cellular processes, including nucleocytoplasmic transport, spindle formation, and postmitotic nuclear envelope reassembly. The cytoplasmic Ran GTPase activating protein RanGAP is critical to establish a functional RanGTP/RanGDP gradient across the nuclear envelope and is associated with the outer surface of the nuclear envelope in metazoan and higher plant cells. Arabidopsis thaliana RanGAP association with the root tip nuclear envelope requires a family of likely plant-specific nucleoporins combining coiled-coil and transmembrane domains (CC-TMD) and WPP domain-interacting proteins (WIPs). Wilms Tumor 1 and WIT2 have been identified as a second family of CC-TMD proteins, structurally similar, yet clearly distinct from the WIP family, that is required for RanGAP nuclear envelop association in root tip cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Evaluating the transcriptional fidelity of cancer models.

Genome medicine

Peng D,Gleyzer R,Tai WH,Kumar P,Bian Q,Isaacs B,da Rocha EL,Cai S,DiNapoli K,Huang FW,Cahan P

Published figure using WT1 monoclonal antibody (Product # MA1-46028) in Immunocytochemistry

Thu Apr 29 00:00:00 EDT 2021

Evaluation of the Preclinical Efficacy of Lurbinectedin in Malignant Pleural Mesothelioma.

Cancers

Anobile DP,Bironzo P,Picca F,Lingua MF,Morena D,Righi L,Napoli F,Papotti MG,Pittaro A,Di Nicolantonio F,Gigliotti C,Bussolino F,Comunanza V,Guerrera F,Sandri A,Leo F,Libener R,Aviles P,Novello S,Taulli R,Scagliotti GV,Riganti C

MA1-46028 was used in Immunohistochemistry to investigated the in vitro susceptibility of malignant pleural mesothelioma to lurbinectedin (PM01183), a marine-derived drug that recently received accelerated approval by the FDA for the treatment of patients with metastatic small cell lung cancer with disease progression on or after platinum-based chemotherapy.

Wed May 12 00:00:00 EDT 2021

Evaluation of the Preclinical Efficacy of Lurbinectedin in Malignant Pleural Mesothelioma.

Cancers

Wed May 12 00:00:00 EDT 2021

SeqStain is an efficient method for multiplexed, spatialomic profiling of human and murine tissues.

Cell reports methods

Rajagopalan A,Venkatesh I,Aslam R,Kirchenbuechler D,Khanna S,Cimbaluk D,Kordower JH,Gupta V

Published figure using WT1 monoclonal antibody (Product # MA1-46028) in Immunocytochemistry

Mon Jun 21 00:00:00 EDT 2021

Amniotic fluid stem cells ameliorate cisplatin-induced acute renal failure through induction of autophagy and inhibition of apoptosis.

Stem cell research & therapy

Minocha E,Sinha RA,Jain M,Chaturvedi CP,Nityanand S

MA1-46028 was used in Flow Cytometry to evaluate the therapeutic efficacy of AFSC in a cisplatin-induced rat model of acute renal failure (ARF) and investigate the underlying mechanisms responsible for their renoprotective effects.

Wed Dec 04 00:00:00 EST 2019

Identification of fatal outcome in a childhood nasopharyngeal carcinoma patient by protein expression profiling.

International journal of oncology

Saeed MEM,Mertens R,Handgretinger R,Efferth T

MA1-46028 was used in Immunohistochemistry-immunofluorescence to conclude that the investigation of rare NPC cases with poor prognosis may provide an improved understanding of the molecular mechanisms involved in refractory tumors and identification of novel potential therapeutic targets for NPC in the future.

Mon Oct 01 00:00:00 EDT 2018

Wilms' tumor 1 protein expression in endometrial adenocarcinoma and endometrial intra-epithelial neoplasia.

The journal of obstetrics and gynaecology research

Atik Y,Cetinkaya Demir B,Ozan H,Baykara S,Usubutun A,Yılmaz Erturk F

MA146028 was used in immunohistochemistry - paraffin section to measure Wilms' tumor 1 protein expression in endometrial adenocarcinoma and endometrial intra-epithelial neoplasia