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Invitrogen
WWC1 Recombinant Rabbit Monoclonal Antibody (1H4L22)
高级验证
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
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WWC1 Antibody (703009) in ICC/IF
Knockdown of WWC1 was achieved by transfecting Caco-2 cells with specific siRNA (Silencer® select Product # s23476, s23478). Immunofluorescence analysis was performed on Caco2 cells (untransfected, panel a-d), transfected with WWC1 specific siRNA (panel i-l) or non-specific scrambled siRNA (panels e-h). Cells were fixed, permeabilized, and labelled with Anti-WWC1 Recombinant Rabbit Monoclonal Antibody (Product # 703009, 1:100 dilution), followed by Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjuga... View More
产品信息
703009
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
1H4L22
抗原
Protein corresponding to human WWC1 [aa772-aa851]
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2762386
产品详细信息
This antibody is predicted to react with Monkey, Dog, Pig.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
WWC1 (Protein KIBRA) is a probable regulator of the Hippo/SWH signaling pathway. This pathway plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. WWC1, along with NF2, can synergistically induce the phosphorylation of LATS1 and LATS2. It acts as a transcriptional coactivator of ESR1 - which plays an essential role in DYNLL1-mediated ESR1 transactivation. WWC1 also functions as a regulatior of collagen-stimulated activation of the ERK/MAPK cascade.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: FLJ10865; FLJ23369; HBeAg-binding protein 3; KIBRA; Kidney and brain protein; Protein KIBRA; protein phosphatase 1, regulatory subunit 168; protein WWC1; WW domain-containing protein 1; WW, C2 and coiled-coil domain containing 1
基因别名: HBEBP3; HBEBP36; KIAA0869; KIBRA; MEMRYQTL; PPP1R168; WWC1
UniProt ID: (Human) Q8IX03
Entrez Gene ID: (Human) 23286
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
cell migration
establishment of cell polarity
regulation of intracellular transport
hippo signaling
regulation of hippo signaling
negative regulation of hippo signaling
positive regulation of MAPK cascade
negative regulation of organ growth
