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Wnt10B Antibody (PA5-116125) in ICC/IF
Immunocytochemistry analysis of Wnt10B in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-116125) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
产品信息
PA5-116125
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human WNT10B(Accession O00744), corresponding to amino acid residues R169-A219.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2900759
产品详细信息
Antibody detects endogenous levels of total Wnt10b.
靶标信息
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. This protein is 96% identical to the mouse Wnt10b protein at the amino acid level. This gene is clustered with another family member, WNT1, in the chromosome 12q13 region.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Protein Wnt-10b; Protein Wnt-12; wingless related MMTV integration site 10b; wingless-type MMTV integration site family member 10B; wingless-type MMTV integration site family, member 10B; WNT-10B protein; wnt-12
基因别名: SHFM6; Wnt-10b; WNT-12; Wnt10; WNT10B; WNT12
UniProt ID: (Human) O00744, (Mouse) P48614
Entrez Gene ID: (Human) 7480, (Mouse) 22410
G2/M transition of mitotic cell cycle
negative regulation of transcription from RNA polymerase II promoter
chondrocyte differentiation
lipid metabolic process
cell cycle arrest
smoothened signaling pathway
positive regulation of cell proliferation
positive regulation of G2/M transition of mitotic cell cycle
myoblast differentiation involved in skeletal muscle regeneration
Wnt signaling pathway
neuron differentiation
positive regulation of bone mineralization
positive regulation of epithelial cell differentiation
regulation of proteasomal ubiquitin-dependent protein catabolic process
positive regulation of apoptotic process
cell fate commitment
negative regulation of fat cell differentiation
positive regulation of osteoblast differentiation
positive regulation of RNA polymerase II transcriptional preinitiation complex assembly
regulation of skeletal muscle tissue development
skeletal muscle fiber development
negative regulation of epithelial cell proliferation
protein stabilization
sensory perception of taste
positive regulation of sequence-specific DNA binding transcription factor activity
positive regulation of anagen
canonical Wnt signaling pathway
bone trabecula formation
fungiform papilla development
cellular response to retinoic acid
cellular response to cAMP
cellular response to parathyroid hormone stimulus
hematopoietic stem cell proliferation
cellular response to hydrostatic pressure
positive regulation of canonical Wnt signaling pathway
regulation of transcription from RNA polymerase II promoter
signal transduction
cell-cell signaling
multicellular organism development
organ morphogenesis
skeletal muscle tissue regeneration
regulation of fat cell differentiation
positive regulation of ossification
positive regulation of transcription from RNA polymerase II promoter
regulation of protein metabolic process
cellular response to organic substance
cellular response to organic cyclic compound
