Invitrogen

XPOT Polyclonal Antibody

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抗体检测数据 (2)
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XPOT Antibody in Immunocytochemistry (ICC/IF)

XPOT Antibody in Immunocytochemistry (ICC/IF)

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XPOT Antibody (PA5-66095) in ICC/IF

Immunofluorescent staining of XPOT in human cell line MCF7 shows localization to nucleoplasm and cytosol. Samples were probed using a XPOT Polyclonal Antibody (Product # PA5-66095).

XPOT Antibody in Immunocytochemistry (ICC/IF)

XPOT Antibody in Western Blot (WB)

XPOT Antibody in Immunocytochemistry (ICC/IF)

Product Image Gallery

XPOT Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent staining of XPOT in human cell line MCF7 shows localization to nucleoplasm and cytosol. Samples were probed using a XPOT Polyclonal Antibody (Product # PA5-66095).

XPOT Antibody in Western Blot (WB) — Western blot analysis of XPOT in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue. Samples were probed using a XPOT Polyclonal Antibody (Product # PA5-66095).

XPOT Antibody in Immunocytochemistry (ICC/IF) — Fig. 4. Identification of importin and exportin for NFAT5 nuclear import and export. (A) In vitro nuclear import assay of His-SV40 NLS -AcGFP and His-NFAT5 171-250 -AcGFP was carried out using digitonin-permeabilized HeLa cells supplemented with individual nuclear transport factors, as indicated. The transport assay was carried out for 30 min at 37degC. Cells were then fixed with paraformaldehyde and stained with DAPI, and GFP fluorescence images were taken using a confocal microscope. Scale bar: 30 um. Images are representative of three independent experiments. (B) Role of KPNB1 in nuclear import of His-NFAT5-AcGFP. Left: schematic representation of the indicated His-NFAT5-AcGFP reporter constructs. Shaded regions indicate NFAT5-cNLS. Right: in vitro nuclear import assay of His-SV40 NLS -AcGFP and the indicated His-NFAT5-AcGFP fusions was carried out using digitonin-permeabilized HeLa cells supplemented with RanGTP, NTF2 and ATP-regenerating mixture, in the presence or absence of KPNB1. The transport assay was carried out for 30 min at 37degC. Cells were then fixed with paraformaldehyde and stained with DAPI, and images were taken using a confocal microscope. Scale bar: 30 um. Images are representative of three independent experiments. (C) Quantitative analysis of subcellular localization of immunofluorescence signal in HeLa cells transfected with FLAG-NFAT5 132-581 and siRNA targeting the indicated members of the exportin family. Cells were switched to isotonic (Iso) or hyp

产品信息

PA5-66095

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.04-0.4 µg/mL

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免疫细胞化学 (ICC/IF)

0.25-2 µg/mL

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant Human XPOT. Recombinant protein control fragment (Product #RP-105080).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2662126

产品详细信息

Immunogen sequence: MDEQALLGLNP NADSDFRQRA LAYFEQLKIS PDAWQVCAEA LAQRTYSDDH VKFFCFQVLE HQVKYKYSEL TTVQQQLIRE TLISWLQAQM LNPQP

Highest antigen sequence identity to the following orthologs - mouse 95%, rat 95%.

靶标信息

XPOT is a protein belonging to the RAN-GTPase exportin family that mediates export of tRNA from the nucleus to the cytoplasm. Translocation of tRNA to the cytoplasm occurs once exportin has bound both tRNA and GTP-bound RAN.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Unconventional tonicity-regulated nuclear trafficking of NFAT5 mediated by KPNB1, XPOT and RUVBL2.

Journal of cell science

Cheung CY,Huang TT,Chow N,Zhang S,Zhao Y,Chau MP,Chan WC,Wong CCL,Boassa D,Phan S,Ellisman MH,Yates JR,Xu S,Yu Z,Zhang Y,Zhang R,Ng LL,Ko BCB

Published figure using XPOT polyclonal antibody (Product # PA5-66095) in Immunocytochemistry

Fri Jul 01 00:00:00 EDT 2022

Unconventional tonicity-regulated nuclear trafficking of NFAT5 mediated by KPNB1, XPOT and RUVBL2.

Journal of cell science

Cheung CY,Huang TT,Chow N,Zhang S,Zhao Y,Chau MP,Chan WC,Wong CCL,Boassa D,Phan S,Ellisman MH,Yates JR,Xu S,Yu Z,Zhang Y,Zhang R,Ng LL,Ko BCB

Published figure using XPOT polyclonal antibody (Product # PA5-66095) in Immunocytochemistry

Fri Jul 01 00:00:00 EDT 2022

Unconventional tonicity-regulated nuclear trafficking of NFAT5 mediated by KPNB1, XPOT and RUVBL2.

Journal of cell science

Cheung CY,Huang TT,Chow N,Zhang S,Zhao Y,Chau MP,Chan WC,Wong CCL,Boassa D,Phan S,Ellisman MH,Yates JR,Xu S,Yu Z,Zhang Y,Zhang R,Ng LL,Ko BCB

Published figure using XPOT polyclonal antibody (Product # PA5-66095) in Immunocytochemistry

Fri Jul 01 00:00:00 EDT 2022

生物信息学

蛋白别名: Exportin(tRNA); exportin, tRNA (nuclear export receptor for tRNAs); Exportin-T; tRNA exportin

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基因别名: XPO3; XPOT

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UniProt ID: (Human) O43592

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Entrez Gene ID: (Human) 11260

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tRNA binding Ran GTPase binding nucleobase-containing compound transmembrane transporter activity RNA metabolism protein

参与通路

tRNA export from nucleus intracellular protein transport tRNA re-export from nucleus