Invitrogen

ZC3H12A Polyclonal Antibody

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抗体检测数据 (3)

ZC3H12A Antibody in Immunocytochemistry (ICC/IF)

ZC3H12A Antibody in Immunocytochemistry (ICC/IF)

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ZC3H12A Antibody (PA5-20926) in ICC/IF

Immunofluorescence analysis of Endoribonuclease ZC3H12A was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with ZC3H12A Polyclonal Antibody (Product # PA5-20926) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (... View More

ZC3H12A Antibody in Immunocytochemistry (ICC/IF)

ZC3H12A Antibody in Western Blot (WB)

ZC3H12A Antibody in Western Blot (WB)

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ZC3H12A Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Endoribonuclease ZC3H12A was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with ZC3H12A Polyclonal Antibody (Product # PA5-20926) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with Hoechst 33342 (Product # H1399). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 40X magnification in CellInsight CX7 LZR High-Content Screening (HCS) Platform (Product # CX7A1110LZR) and externally deconvoluted (D.Sage et al. / Methods 115 (2017) 28-41).

ZC3H12A Antibody in Western Blot (WB) — Western blot was performed using Anti-ZC3H12A Polyclonal Antibody (Product # PA5-20926) and a ~65 kDa band corresponding to Endoribonuclease ZC3H12A was observed across cell lines tested. Whole cell extracts (50 µg lysate) of THP-1 (Lane 1), THP-1 treated with 5 ng/mL PMA for 48h along with 100 ng/mL LPS for 12h (Lane 2), THP-1 treated with 100 ng/mL LPS for 12h (Lane 3), RAW 264.7 (Lane 4), RAW 264.7 treated with 100 ng/mL LPS for 12h (Lane 5), SK-O-V3 (Lane 6), Raji (Lane 7), SH-SY5Y (Lane 8), IMR-32 (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).a non specific band was observed around 62 kDa across cell lines tested.

ZC3H12A Antibody in Western Blot (WB) — Western Blot analysis of ZC3H12A in K562 cell lysate with ZC3H12A Polyclonal Antibody (Product # PA5-20926) at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide.

产品信息

PA5-20926

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

-

免疫细胞化学 (ICC/IF)

1:100

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A 17 amino acid peptide near the center of human ZC3H12A.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

0.02% sodium azide

保存条件

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_11153279

产品详细信息

A suggested positive control is Raji cell lysate.

PA5-20926 can be used with blocking peptide PEP-1040.

靶标信息

ZC3H12A is an MCP1 -induced protein that acts as a transcriptional activator and causes cell death of cardiomyocytes, possibly via induction of genes associated with apoptosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Endoribonuclease ZC3H12A; MCP-1 treatment-induced protein; MCP-induced protein 1; Monocyte chemotactic protein-induced protein 1; Reg1; Regnase-1; ribonuclease ZC3H12A; RP3-423B22.1; zc3h12a; Zinc finger CCCH domain-containing protein 12A

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基因别名: BC036563; dJ423B22.1; MCPIP; MCPIP1; ZC3H12A

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UniProt ID: (Human) Q5D1E8, (Mouse) Q5D1E7

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Entrez Gene ID: (Human) 80149, (Mouse) 230738

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DNA binding chromatin binding RNA binding mRNA binding mRNA 3'-UTR binding endoribonuclease activity exoribonuclease activity ribonuclease activity thiol-dependent ubiquitin-specific protease activity protein binding miRNA binding RNA stem-loop binding mRNA 3'-UTR AU-rich region binding ribosome binding metal ion binding nuclease activity endonuclease activity hydrolase activity phosphoric ester hydrolase activity T/G mismatch-specific endonuclease activity retroviral 3' processing activity endoribonuclease

参与通路

nuclear-transcribed mRNA catabolic process, endonucleolytic cleavage-dependent decay angiogenesis negative regulation of protein phosphorylation positive regulation of defense response to virus by host transcription, DNA-templated apoptotic process inflammatory response cellular response to DNA damage stimulus nervous system development regulation of gene expression positive regulation of autophagy positive regulation of endothelial cell migration positive regulation of gene expression negative regulation of gene expression negative regulation of muscle cell apoptotic process positive regulation of lipid storage positive regulation of cell death protein deubiquitination cell differentiation negative regulation of NF-kappaB transcription factor activity negative regulation of interleukin-6 production negative regulation of tumor necrosis factor production cellular response to oxidative stress cellular response to glucose starvation positive regulation of protein import into nucleus negative regulation of NF-kappaB import into nucleus negative regulation of macrophage activation negative regulation of I-kappaB kinase/NF-kappaB signaling negative regulation by host of viral genome replication negative regulation of nitric oxide biosynthetic process positive regulation of fat cell differentiation positive regulation of angiogenesis positive regulation of transcription from RNA polymerase II promoter negative regulation of interleukin-1 beta secretion protein oligomerization defense response to virus negative regulation of cardiac muscle contraction positive regulation of mRNA catabolic process 3'-UTR-mediated mRNA destabilization cellular response to lipopolysaccharide cellular response to interleukin-1 cellular response to tumor necrosis factor RNA phosphodiester bond hydrolysis RNA phosphodiester bond hydrolysis, endonucleolytic RNA phosphodiester bond hydrolysis, exonucleolytic cellular response to virus negative regulation of cytokine production involved in inflammatory response positive regulation of execution phase of apoptosis negative regulation of interleukin-6 secretion positive regulation of p38MAPK cascade negative regulation of interferon-gamma secretion positive regulation of protein deubiquitination negative regulation of production of miRNAs involved in gene silencing by miRNA cellular response to sodium arsenite negative regulation of tumor necrosis factor secretion cellular response to phorbol 13-acetate 12-myristate cellular response to ionomycin cellular response to chemokine positive regulation of reactive oxygen species metabolic process positive regulation of miRNA catabolic process multicellular organism development