Invitrogen

ZC3H12A Polyclonal Antibody

查看其他7个ZC3H12A抗体

抗体检测数据 (6)

ZC3H12A Antibody in Immunocytochemistry (ICC/IF)

ZC3H12A Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 6

ZC3H12A Antibody (PA5-22137) in ICC/IF

ZC3H12A antibody detects ZC3H12A protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZC3H12A stained by ZC3H12A antibody (Product # PA5-22137) diluted at 1:500.

ZC3H12A Antibody in Immunocytochemistry (ICC/IF)

ZC3H12A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ZC3H12A Antibody in Western Blot (WB)

ZC3H12A Antibody in Western Blot (WB)

ZC3H12A Antibody in Western Blot (WB)

ZC3H12A Antibody in Western Blot (WB)

Product Image Gallery

ZC3H12A Antibody in Immunocytochemistry (ICC/IF) — ZC3H12A antibody detects ZC3H12A protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZC3H12A stained by ZC3H12A antibody (Product # PA5-22137) diluted at 1:500.

ZC3H12A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of ZC3H12A was performed in paraffin-embedded mouse kidney tissue using ZC3H12A Polyclonal Antibody (Product # PA5-22137) at a dilution of 1:250.

ZC3H12A Antibody in Western Blot (WB) — Western blot was performed using Anti-ZC3H12A Polyclonal Antibody (Product # PA5-22137) and a ~65 kDa band corresponding to Endoribonuclease ZC3H12A was observed across cell lines and tissues tested . Whole cell extracts (30 µg lysate) of THP-1 (Lane 1), THP-1 treated with 1 µg/mL LPS for 16h (Lane 2), SK-O-V3 (Lane 3), Raji (Lane 4), SH-SY5Y (Lane 5), IMR-32 (Lane 6), Mouse Heart (Lane 7), were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20000 using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).Signal was induced in THP-1 cells treated with LPS as seen in lanes 1 and 2.

ZC3H12A Antibody in Western Blot (WB) — Knockdown of Endoribonuclease ZC3H12A was achieved by transfecting Raji with Endoribonuclease ZC3H12A specific siRNAs (Silencer® select Product # s36970, s36971). Western blot analysis (Fig. a) was performed using Whole cell extracts from the Endoribonuclease ZC3H12A knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with ZC3H12A Polyclonal Antibody (Product # PA5-22137, 1:1000 ) and Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20000). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Endoribonuclease ZC3H12A.

ZC3H12A Antibody in Western Blot (WB) — Western Blot using ZC3H12A Polyclonal Antibody (Product # PA5-22137). Untreated (–) and treated (+) THP-1 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZC3H12A Polyclonal Antibody (Product # PA5-22137) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

ZC3H12A Antibody in Western Blot (WB) — Western Blot analysis of ZC3H12A was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ZC3H12A Polyclonal Antibody (Product # PA5-22137) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

产品信息

PA5-22137

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

-

免疫组化（石蜡） (IHC (P))

Assay-dependent

-

免疫细胞化学 (ICC/IF)

1:100-1:1,000

-

流式细胞分析 (Flow)

Assay-dependent

-

免疫沉淀 (IP)

Assay-dependent

-

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 241 and 504 of ZC3H12A (Uniprot ID#Q5D1E8)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_11155824

产品详细信息

Recommended positive controls: 293T, A431.

Predicted reactivity: Bovine (83%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

ZC3H12A is an MCP1 -induced protein that acts as a transcriptional activator and causes cell death of cardiomyocytes, possibly via induction of genes associated with apoptosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Endoribonuclease ZC3H12A; MCP-1 treatment-induced protein; MCP-induced protein 1; Monocyte chemotactic protein-induced protein 1; Reg1; Regnase-1; ribonuclease ZC3H12A; RP3-423B22.1; Zinc finger CCCH domain-containing protein 12A

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基因别名: dJ423B22.1; MCPIP; MCPIP1; ZC3H12A

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UniProt ID: (Human) Q5D1E8

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Entrez Gene ID: (Human) 80149

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DNA binding chromatin binding RNA binding mRNA binding mRNA 3'-UTR binding endoribonuclease activity exoribonuclease activity ribonuclease activity thiol-dependent ubiquitin-specific protease activity protein binding miRNA binding RNA stem-loop binding mRNA 3'-UTR AU-rich region binding ribosome binding metal ion binding endoribonuclease

参与通路

nuclear-transcribed mRNA catabolic process, endonucleolytic cleavage-dependent decay angiogenesis negative regulation of protein phosphorylation positive regulation of defense response to virus by host transcription, DNA-templated apoptotic process inflammatory response cellular response to DNA damage stimulus nervous system development regulation of gene expression positive regulation of autophagy positive regulation of endothelial cell migration positive regulation of gene expression negative regulation of gene expression negative regulation of muscle cell apoptotic process positive regulation of lipid storage positive regulation of cell death protein deubiquitination cell differentiation negative regulation of NF-kappaB transcription factor activity negative regulation of interleukin-6 production negative regulation of tumor necrosis factor production cellular response to oxidative stress cellular response to glucose starvation positive regulation of protein import into nucleus negative regulation of NF-kappaB import into nucleus negative regulation of macrophage activation negative regulation of I-kappaB kinase/NF-kappaB signaling negative regulation by host of viral genome replication negative regulation of nitric oxide biosynthetic process positive regulation of fat cell differentiation positive regulation of angiogenesis positive regulation of transcription from RNA polymerase II promoter negative regulation of interleukin-1 beta secretion protein oligomerization defense response to virus negative regulation of cardiac muscle contraction positive regulation of mRNA catabolic process 3'-UTR-mediated mRNA destabilization cellular response to lipopolysaccharide cellular response to interleukin-1 cellular response to tumor necrosis factor RNA phosphodiester bond hydrolysis RNA phosphodiester bond hydrolysis, endonucleolytic RNA phosphodiester bond hydrolysis, exonucleolytic cellular response to virus negative regulation of cytokine production involved in inflammatory response positive regulation of execution phase of apoptosis negative regulation of interleukin-6 secretion positive regulation of p38MAPK cascade negative regulation of interferon-gamma secretion positive regulation of protein deubiquitination negative regulation of production of miRNAs involved in gene silencing by miRNA cellular response to sodium arsenite negative regulation of tumor necrosis factor secretion cellular response to phorbol 13-acetate 12-myristate cellular response to ionomycin cellular response to chemokine positive regulation of reactive oxygen species metabolic process positive regulation of miRNA catabolic process