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ZNF143 Antibody (PA5-116147) in ICC/IF
Immunocytochemistry analysis of ZNF143 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-116147) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
产品信息
PA5-116147
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Not Applicable
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human ZNF143(Accession P52747), corresponding to amino acid residues I73-S123.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2900781
产品详细信息
Antibody detects endogenous levels of total ZNF143.
靶标信息
Transcriptional activator. Activates the gene for selenocysteine tRNA (tRNAsec). Binds to the SPH motif of small nuclear RNA (snRNA) gene promoters. Participates in efficient U6 RNA polymerase III transcription via its interaction with CHD8.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: hStaf; mStaf; selenocysteine tRNA gene transcription activating factor; Selenocysteine tRNA gene transcription-activating factor; SPH-binding factor; transcriptional activator Staf; Zfp-143; Zinc finger protein 143; zinc finger protein 80, related sequence 1
基因别名: AA959806; AU015869; D7Ertd805e; KRAB14; pHZ-1; SBF; STAF; Zfp143; Zfp79; Zfp80-rs1; ZNF143
UniProt ID: (Human) P52747, (Mouse) O70230, (Rat) Q5XIU2
Entrez Gene ID: (Human) 7702, (Mouse) 20841, (Rat) 361627
regulation of transcription from RNA polymerase II promoter
regulation of transcription from RNA polymerase III promoter
transcription from RNA polymerase II promoter
transcription from RNA polymerase III promoter
snRNA transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of smooth muscle cell proliferation
