Invitrogen

ZO-1 Polyclonal Antibody

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

8个已发表图片

12 篇参考文献

查看其他33个ZO-1抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

ZO-1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 17

ZO-1 Antibody (PA5-28858) in ICC/IF

Immunofluorescence analysis of ZO-1 was performed using 90% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with ZO-1 Polyclonal Antibody (Product # PA5-28858) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A327... View More

ZO-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ZO-1 Antibody in Immunohistochemistry (IHC)

ZO-1 Antibody in Western Blot, Immunocytochemistry, Immunohistochemistry (WB, ICC/IF, IHC)

ZO-1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Product Image Gallery

ZO-1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. Caco-2 cells were transfected with ZO-1 siRNA and decrease in signal intensity was observed in ICC application using Anti-ZO-1 Polyclonal Antibody (Product # PA5-28858). {KD}

产品信息

PA5-28858

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

查看4篇已发表文章 4篇已发表文章

免疫组化 (IHC)

查看5篇已发表文章 5篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

查看2篇已发表文章 2篇已发表文章

免疫组化（冰冻） (IHC (F))

Assay-dependent

免疫细胞化学 (ICC/IF)

Assay-dependent

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Bovine, Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 373 and 615 of Human ZO-1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.19 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol, 1% BSA

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2546334

产品详细信息

Recommended positive controls: 293T, A431, HeLa, HepG2, A549, H1299, HCT116.

Predicted reactivity: Mouse (100%), Rat (99%), Dog (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Curcumin reduces blood-nerve barrier abnormalities and cytotoxicity to endothelial cells and pericytes induced by cisplatin.

Folia morphologica

Kobutree P,Tothonglor A,Roumwong A,Jindatip D,Agthong S

PA5-28858 was used in Western Blotting to investigate if cisplatin causes blood-nerve barrier disruption in the nerves and might result in neuropathy.

Wed Oct 18 00:00:00 EDT 2023

Glycine-β-Muricholic Acid Improves Liver Fibrosis and Gut Barrier Function by Reducing Bile Acid Pool Size and Hydrophobicity in Male Cyp2c70 Knockout Mice.

Cells

Hasan MN,Chen J,Wang H,Du Y,Clayton YD,Gu L,Li T

PA5-28858 was used in Immunohistochemistry to investigate the potential anti-cholestasis effect of glycine-conjugated β muricholic acid (G-β-MCA) in male Cyp2c70 KO mice based on its hydrophilic physiochemical property and signaling property as an farnesoid X receptor (FXR) antagonist.

Fri May 12 00:00:00 EDT 2023

Glycine-β-Muricholic Acid Improves Liver Fibrosis and Gut Barrier Function by Reducing Bile Acid Pool Size and Hydrophobicity in Male Cyp2c70 Knockout Mice.

Cells

Hasan MN,Chen J,Wang H,Du Y,Clayton YD,Gu L,Li T

Fri May 12 00:00:00 EDT 2023

Acetate and propionate effects in response to LPS in a porcine intestinal co-culture model.

Porcine health management

Andrani M,Borghetti P,Ravanetti F,Cavalli V,Ferrari L,De Angelis E,Martelli P,Saleri R

Published figure using ZO-1 polyclonal antibody (Product # PA5-28858) in Western Blot

Tue May 23 00:00:00 EDT 2023

Combining ASBT inhibitor and FGF15 treatments enhances therapeutic efficacy against cholangiopathy in female but not male Cyp2c70 KO mice.

Journal of lipid research

Hasan MN,Chen J,Matye D,Wang H,Luo W,Gu L,Clayton YD,Du Y,Li T

PA5-28858 was used in Immunohistochemistry to conclude that combined ASBT inhibitor and FGF15 treatment improved therapeutic efficacy against cholangiopathy than either single treatment in the female but not male Cyp2c70 KO mice by reducing bile acid pool size and hydrophobicity.

Wed Mar 01 00:00:00 EST 2023

Combining ASBT inhibitor and FGF15 treatments enhances therapeutic efficacy against cholangiopathy in female but not male Cyp2c70 KO mice.

Journal of lipid research

Hasan MN,Chen J,Matye D,Wang H,Luo W,Gu L,Clayton YD,Du Y,Li T

Wed Mar 01 00:00:00 EST 2023

miR-4456/CCL3/CCR5 Pathway in the Pathogenesis of Tight Junction Impairment in Chronic Obstructive Pulmonary Disease.

Frontiers in pharmacology

Yu W,Ye T,Ding J,Huang Y,Peng Y,Xia Q,Cuntai Z

Published figure using ZO-1 polyclonal antibody (Product # PA5-28858) in Immunocytochemistry

Sat May 08 00:00:00 EDT 2021

miR-4456/CCL3/CCR5 Pathway in the Pathogenesis of Tight Junction Impairment in Chronic Obstructive Pulmonary Disease.

Frontiers in pharmacology

Yu W,Ye T,Ding J,Huang Y,Peng Y,Xia Q,Cuntai Z

Published figure using ZO-1 polyclonal antibody (Product # PA5-28858) in Immunocytochemistry

Sat May 08 00:00:00 EDT 2021

miR-4456/CCL3/CCR5 Pathway in the Pathogenesis of Tight Junction Impairment in Chronic Obstructive Pulmonary Disease.

Frontiers in pharmacology

Yu W,Ye T,Ding J,Huang Y,Peng Y,Xia Q,Cuntai Z

Published figure using ZO-1 polyclonal antibody (Product # PA5-28858) in Immunocytochemistry

Sat May 08 00:00:00 EDT 2021

Hyaluronan 35 kDa enhances epithelial barrier function and protects against the development of murine necrotizing enterocolitis.

Pediatric research

Gunasekaran A,Eckert J,Burge K,Zheng W,Yu Z,Kessler S,de la Motte C,Chaaban H

PA5-28858 was used in Immunohistochemistry (Paraffin) to investigate the effects of HA 35 kDa on a NEC-like murine model.

Mon Jun 01 00:00:00 EDT 2020

Protective effect of Saccharomyces boulardii on intestinal mucosal barrier of dextran sodium sulfate-induced colitis in mice.

Chinese medical journal

Dong JP,Zheng Y,Wu T,He Q,Teng GG,Wang HH

PA5-28858 was used in Immunohistochemistry to study the effect of Saccharomyces boulardii on intestinal mucosal mechanical barrier of which included an anti-inflammatory and protective effect.

Tue Aug 20 00:00:00 EDT 2019

Anesthesia and Surgery Impair Blood-Brain Barrier and Cognitive Function in Mice.

Frontiers in immunology

Yang S,Gu C,Mandeville ET,Dong Y,Esposito E,Zhang Y,Yang G,Shen Y,Fu X,Lo EH,Xie Z

PA5-28858 was used in Western Blotting to promote mechanistic studies of postoperative cognitive impairment, including postoperative delirium.

Mon Aug 07 00:00:00 EDT 2023

RFX2 Is a Major Transcriptional Regulator of Spermiogenesis.

PLoS genetics

Kistler WS,Baas D,Lemeille S,Paschaki M,Seguin-Estevez Q,Barras E,Ma W,Duteyrat JL,Morlé L,Durand B,Reith W

Published figure using ZO-1 polyclonal antibody (Product # PA5-28858) in Immunofluorescence

Wed Jul 01 00:00:00 EDT 2015

生物信息学

蛋白别名: DKFZp686M05161; MGC133289; OTTHUMP00000174520; Tight junction protein 1; Tight junction protein ZO-1; zona occludens 1; Zona occludens protein 1; zonula occludens 1 protein; Zonula occludens protein 1

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基因别名: TJP1; ZO-1; ZO1

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UniProt ID: (Human) Q07157, (Mouse) P39447

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Entrez Gene ID: (Bovine) 407102, (Human) 7082, (Mouse) 21872

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功能

protein binding calmodulin binding protein domain specific binding cadherin binding involved in cell-cell adhesion protein C-terminus binding tight junction

参与通路

blastocyst formation cell-cell junction assembly sensory perception of sound hippo signaling establishment of endothelial intestinal barrier cell-cell adhesion cell-cell signaling involved in cell-cell junction organization