Proteintech

ZRANB3 Polyclonal Antibody

ZRANB3 Antibody in Immunocytochemistry (ICC/IF)

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ZRANB3 Antibody (23111-1-AP) in ICC/IF

Immunofluorescent analysis of HEK-293 cells using 23111-1-AP (ZRANB3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

ZRANB3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

23111-1-AP

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

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免疫组化（石蜡） (IHC (P))

1:50-1:500

免疫细胞化学 (ICC/IF)

1:20-1:200

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

ZRANB3 Fusion Protein Ag19407 (728-1079 aa encoded by BC064616)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.35 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice (domestic); Dry ice (international)

产品详细信息

Immunogen sequence: LPVYDTLMF CASRNTDRIH IYTKDGKQMS CNFIPLDIKL DLWEDLPASF QLKQYRSLIL RFVREWSSLT AMKQRIIRKS GQLFCSPILA LEEITKQQTK QNCTKRYITK EDVAVASMDK VKNVGGHVRL ITKESRPRDP FTKKLLEDGA CVPFLNPYTV QADLTVKPST SKGYLQAVDN EGNPLCLRCQ QPTCQTKQAC KANSWDSRFC SLKCQEEFWI RSNNSYLRAK VFETEHGVCQ LCNVNAQELF LRLRDAPKSQ RKNLLYATWT SKLPLEQLNE MIRNPGEGHF WQVDHIKPVY GGGGQCSLDN LQTLCTVCHK ERTARQAKER SQVRRQSLAS KHGSDITRFL VKK (728-1079 aa encoded by B C064616)

靶标信息

DNA annealing helicase and endonuclease required to maintain genome stability at stalled or collapsed replication forks by facilitating fork restart and limiting inappropriate recombination that could occur during template switching events (PubMed:21078962, PubMed:22704558, PubMed:22705370, PubMed:22759634, PubMed:26884333). Recruited to the sites of stalled DNA replication by polyubiquitinated PCNA and acts as a structure-specific endonuclease that cleaves the replication fork D-loop intermediate, generating an accessible 3'-OH group in the template of the leading strand, which is amenable to extension by DNA polymerase (PubMed:22759634). In addition to endonuclease activity, also catalyzes the fork regression via annealing helicase activity in order to prevent disintegration of the replication fork and the formation of double-strand breaks (PubMed:22705370, PubMed:22704558). [UniProt]

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

RFWD3 promotes ZRANB3 recruitment to regulate the remodeling of stalled replication forks.

The Journal of cell biology

Moore CE,Yalcindag SE,Czeladko H,Ravindranathan R,Wijesekara Hanthi Y,Levy JC,Sannino V,Schindler D,Ciccia A,Costanzo V,Elia AEH

Mon May 01 00:00:00 EDT 2023

Excessive reactive oxygen species induce transcription-dependent replication stress.

Nature communications

Andrs M,Stoy H,Boleslavska B,Chappidi N,Kanagaraj R,Nascakova Z,Menon S,Rao S,Oravetzova A,Dobrovolna J,Surendranath K,Lopes M,Janscak P

Thu Mar 30 00:00:00 EDT 2023

RAD52 prevents accumulation of Polα-dependent replication gaps at perturbed replication forks in human cells.

bioRxiv : the preprint server for biology

Di Biagi L,Malacaria E,Aiello FA,Valenzisi P,Marozzi G,Franchitto A,Pichierri P

Wed Apr 12 00:00:00 EDT 2023

Active DNA damage eviction by HLTF stimulates nucleotide excision repair.

Molecular cell

van Toorn M,Turkyilmaz Y,Han S,Zhou D,Kim HS,Salas-Armenteros I,Kim M,Akita M,Wienholz F,Raams A,Ryu E,Kang S,Theil AF,Bezstarosti K,Tresini M,Giglia-Mari G,Demmers JA,Schärer OD,Choi JH,Vermeulen W,Marteijn JA

Thu Apr 07 00:00:00 EDT 2022

DDX17 helicase promotes resolution of R-loop-mediated transcription-replication conflicts in human cells.

Nucleic acids research

Boleslavska B,Oravetzova A,Shukla K,Nascakova Z,Ibini ON,Hasanova Z,Andrs M,Kanagaraj R,Dobrovolna J,Janscak P

Mon Nov 28 00:00:00 EST 2022

p53 isoforms differentially impact on the POLι dependent DNA damage tolerance pathway.

Cell death & disease

Guo Y,Rall-Scharpf M,Bourdon JC,Wiesmüller L,Biber S

Wed Oct 13 00:00:00 EDT 2021

BRCA2 associates with MCM10 to suppress PRIMPOL-mediated repriming and single-stranded gap formation after DNA damage.

Nature communications

Kang Z,Fu P,Alcivar AL,Fu H,Redon C,Foo TK,Zuo Y,Ye C,Baxley R,Madireddy A,Buisson R,Bielinsky AK,Zou L,Shen Z,Aladjem MI,Xia B

Wed Oct 13 00:00:00 EDT 2021

Fork Cleavage-Religation Cycle and Active Transcription Mediate Replication Restart after Fork Stalling at Co-transcriptional R-Loops.

Molecular cell

Chappidi N,Nascakova Z,Boleslavska B,Zellweger R,Isik E,Andrs M,Menon S,Dobrovolna J,Balbo Pogliano C,Matos J,Porro A,Lopes M,Janscak P

Thu Feb 06 00:00:00 EST 2020

Sequential role of RAD51 paralog complexes in replication fork remodeling and restart.

Nature communications

Berti M,Teloni F,Mijic S,Ursich S,Fuchs J,Palumbieri MD,Krietsch J,Schmid JA,Garcin EB,Gon S,Modesti M,Altmeyer M,Lopes M

Wed Jul 15 00:00:00 EDT 2020

Rad52 prevents excessive replication fork reversal and protects from nascent strand degradation.

Nature communications

Malacaria E,Pugliese GM,Honda M,Marabitti V,Aiello FA,Spies M,Franchitto A,Pichierri P

Fri Mar 29 00:00:00 EDT 2019

WRNIP1 Protects Reversed DNA Replication Forks from SLX4-Dependent Nucleolytic Cleavage.

iScience

Porebski B,Wild S,Kummer S,Scaglione S,Gaillard PL,Gari K

Fri Nov 22 00:00:00 EST 2019

CtIP-Mediated Fork Protection Synergizes with BRCA1 to Suppress Genomic Instability upon DNA Replication Stress.

Molecular cell

Przetocka S,Porro A,Bolck HA,Walker C,Lezaja A,Trenner A,von Aesch C,Himmels SF,D'Andrea AD,Ceccaldi R,Altmeyer M,Sartori AA

Thu Nov 01 00:00:00 EDT 2018

CtIP-Mediated Fork Protection Synergizes with BRCA1 to Suppress Genomic Instability upon DNA Replication Stress.

Molecular cell

Przetocka S,Porro A,Bolck HA,Walker C,Lezaja A,Trenner A,von Aesch C,Himmels SF,D'Andrea AD,Ceccaldi R,Altmeyer M,Sartori AA

Thu Nov 01 00:00:00 EDT 2018

BET Inhibition Induces HEXIM1- and RAD51-Dependent Conflicts between Transcription and Replication.

Cell reports

Bowry A,Piberger AL,Rojas P,Saponaro M,Petermann E

Tue Nov 20 00:00:00 EST 2018

BET Inhibition Induces HEXIM1- and RAD51-Dependent Conflicts between Transcription and Replication.

Cell reports

Bowry A,Piberger AL,Rojas P,Saponaro M,Petermann E

Tue Nov 20 00:00:00 EST 2018

Replication Fork Slowing and Reversal upon DNA Damage Require PCNA Polyubiquitination and ZRANB3 DNA Translocase Activity.

Molecular cell

Vujanovic M,Krietsch J,Raso MC,Terraneo N,Zellweger R,Schmid JA,Taglialatela A,Huang JW,Holland CL,Zwicky K,Herrador R,Jacobs H,Cortez D,Ciccia A,Penengo L,Lopes M

Thu Sep 07 00:00:00 EDT 2017

Replication Fork Slowing and Reversal upon DNA Damage Require PCNA Polyubiquitination and ZRANB3 DNA Translocase Activity.

Molecular cell

Vujanovic M,Krietsch J,Raso MC,Terraneo N,Zellweger R,Schmid JA,Taglialatela A,Huang JW,Holland CL,Zwicky K,Herrador R,Jacobs H,Cortez D,Ciccia A,Penengo L,Lopes M

Thu Sep 07 00:00:00 EDT 2017

Replication fork reversal triggers fork degradation in BRCA2-defective cells.

Nature communications

Mijic S,Zellweger R,Chappidi N,Berti M,Jacobs K,Mutreja K,Ursich S,Ray Chaudhuri A,Nussenzweig A,Janscak P,Lopes M

Mon Oct 16 00:00:00 EDT 2017

Replication fork reversal triggers fork degradation in BRCA2-defective cells.

Nature communications

Mijic S,Zellweger R,Chappidi N,Berti M,Jacobs K,Mutreja K,Ursich S,Ray Chaudhuri A,Nussenzweig A,Janscak P,Lopes M

Mon Oct 16 00:00:00 EDT 2017

生物信息学

蛋白别名: AH2; Annealing helicase 2; DNA annealing helicase and endonuclease ZRANB3; Zinc finger Ran-binding domain-containing protein 3; zinc finger, RAN-binding domain containing 3

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基因别名: 4933425L19Rik; AH2; ZRANB3

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UniProt ID: (Human) Q5FWF4

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Entrez Gene ID: (Human) 84083

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功能

DNA helicase activity endodeoxyribonuclease activity protein binding ATP binding zinc ion binding annealing helicase activity K63-linked polyubiquitin binding DNA metabolism protein

参与通路

DNA strand renaturation DNA repair cellular response to DNA damage stimulus response to UV replication fork processing DNA rewinding negative regulation of DNA recombination replication fork protection nucleic acid phosphodiester bond hydrolysis