Invitrogen

alpha-Cardiac Actin Polyclonal Antibody

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

4个已发表图片

8 篇参考文献

查看其他7个alpha-Cardiac Actin抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

alpha-Cardiac Actin Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 15

alpha-Cardiac Actin Antibody (PA5-21396) in ICC/IF

alpha Actin (cardiac muscle) antibody detects alpha Actin (cardiac muscle) protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in 0.5% Triton X-100 for 1 min, then ice-cold methanol for 5 min. Green: alpha Actin (cardiac muscle) protein stained by alpha Actin (cardiac muscle) antibody (Product # PA5-21396) diluted at 1:200. Blue: Hoechst 33342 staining.

alpha-Cardiac Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

alpha-Cardiac Actin Antibody in Western Blot (WB)

alpha-Cardiac Actin Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

alpha-Cardiac Actin Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

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产品信息

PA5-21396

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:10,000

查看4篇已发表文章 4篇已发表文章

免疫组化 (IHC)

查看3篇已发表文章 3篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein fragment contain a sequence corresponding to a region within amino acids 99 and 354 (P68032) of alpha Actin (cardiac muscle)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.39 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_11152296

产品详细信息

Recommended positive controls: Jurkat , Raji, K562, THP-1, NIH-3T3, JC, BCL-1, Rat muscle, PC-12, Rat2.^M
^M
Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (99%), Pig (100%), Chicken (100%), Bovine (100%).^M
^M
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

ACTC1 are part of actins, which are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. The protein encoded by this gene belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in this gene have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Identification of Ferroptosis-Associated Genes in Prostate Cancer by Bioinformatics Analysis.

Frontiers in genetics

Wo Q,Liu Z,Hu L

PA5-21396 was used in Western Blotting to research the influence of ferroptosis on gene mutations, various cellular signaling pathways, biochemical recurrence, and drug resistance in both FPI-high and FPI-low groups.

Fri Jul 22 00:00:00 EDT 2022

Induction of apoptosis and autosis in cardiomyocytes by the combination of homocysteine and copper via NOX-mediated p62 expression.

Cell death discovery

Yin R,Wang H,Li C,Wang L,Lai S,Yang X,Hong D,Zhang W

PA5-21396 was used in Immunohistochemistry to demonstrate that the combination of Hcy and Cu2+-induced apoptosis and autosis of cardiomyocytes simultaneously.

Mon Feb 21 00:00:00 EST 2022

Influence of cytoskeleton organization on recombinant protein expression by CHO cells.

Biotechnology and bioengineering

Pourcel L,Buron F,Arib G,Le Fourn V,Regamey A,Bodenmann I,Girod PA,Mermod N

Published figure using alpha-Cardiac Actin polyclonal antibody (Product # PA5-21396) in Western Blot

Wed Apr 01 00:00:00 EDT 2020

Lymphocyte-specific protein 1 regulates mechanosensory oscillation of podosomes and actin isoform-based actomyosin symmetry breaking.

Nature communications

Cervero P,Wiesner C,Bouissou A,Poincloux R,Linder S

Published figure using alpha-Cardiac Actin polyclonal antibody (Product # PA5-21396) in Immunohistochemistry

Tue Feb 06 00:00:00 EST 2018

Lymphocyte-specific protein 1 regulates mechanosensory oscillation of podosomes and actin isoform-based actomyosin symmetry breaking.

Nature communications

Cervero P,Wiesner C,Bouissou A,Poincloux R,Linder S

Published figure using alpha-Cardiac Actin polyclonal antibody (Product # PA5-21396) in Immunohistochemistry

Tue Feb 06 00:00:00 EST 2018

Lymphocyte-specific protein 1 regulates mechanosensory oscillation of podosomes and actin isoform-based actomyosin symmetry breaking.

Nature communications

Cervero P,Wiesner C,Bouissou A,Poincloux R,Linder S

Published figure using alpha-Cardiac Actin polyclonal antibody (Product # PA5-21396) in Immunohistochemistry

Tue Feb 06 00:00:00 EST 2018

Effects of Ginkgo biloba leaf extract on local renin-angiotensin system through TLR4/NF-κB pathway in cardiac myocyte.

Experimental and therapeutic medicine

Jiang H,Qu P

PA5-21396 was used in Immunohistochemistry to investigate the effects of Ginkgo biloba leaf extract (GBE50) on lipopolysaccharide (LPS) induced Toll-like receptor 4 (TLR4)/nuclear factor-κB (NF-κB) signaling pathway and its effects on angiotensinogen (ATG) and AT1a receptor, so as to explore the mechanism of GBE50 in prevention and treatment of left ventricular remodeling.

Fri Dec 01 00:00:00 EST 2017

Ascribing novel functions to the sarcomeric protein, myosin binding protein H (MyBPH) in cardiac sarcomere contraction.

Experimental cell research

Mouton J,Loos B,Moolman-Smook JC,Kinnear CJ

PA5-21396 was used in immunocytochemistry, immunoprecipitation, and western blot to investigate the role of myosin binding protein H in hypertrophic cardiomyopathy

Sun Feb 15 00:00:00 EST 2015

生物信息学

蛋白别名: actin alpha cardiac; actin alpha cardiac 1; Actin, alpha cardiac muscle 1; actin, alpha, cardiac 1; alpha-actin cardiac; Alpha-cardiac actin; alphac-actin

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基因别名: ACTC; Actc-1; ACTC1; ASD5; CMD1R; CMH11; LVNC4

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UniProt ID: (Human) P68032, (Mouse) P68033, (Rat) P68035

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Entrez Gene ID: (Human) 70, (Mouse) 11464, (Rat) 29275

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功能

ATP binding ATPase activity myosin binding nucleotide binding protein binding actin and actin related protein actin or actin-binding cytoskeletal protein cytoskeletal protein

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