Invitrogen

alpha Catenin Monoclonal Antibody (1G5)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

3个已发表图片

7 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

alpha Catenin Antibody in Immunocytochemistry (ICC/IF)

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alpha Catenin Antibody (MA1-2000) in ICC/IF

Immunofluorescent analysis of alpha Catenin using Catenin alpha Monoclonal Antibody (1G5) (Product # MA1-2000) shows staining in A2058 Cells. Catenin alpha (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Catenin alpha (Pro... View More

alpha Catenin Antibody in Western Blot (WB)

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产品信息

MA1-2000

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100-1:1,000

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免疫细胞化学 (ICC/IF)

10 µg/mL

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免疫沉淀 (IP)

Assay-dependent

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产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

1G5

抗原

90 kDa MBP/alpha catenin fusion protein purified on amylose column.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_326077

产品详细信息

MA1-2000 detects alpha catenin from human samples.

MA1-2000 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~102 kDa protein representing alpha catenin from scc-1 cell extract. In Western blot, this antibody did not cross-react with mouse samples. MA1-2000 can also be used in immunoprecipitation and immunofluorescence procedures.

The MA1-2000 immunogen is a 90 kDa MBP/alpha catenin fusion protein purified via an amylose column.

靶标信息

Alpha catenin is a member of the b-catenin family of cytosolic proteins. Alpha catenin interacts with N-cadherin and b-catenin on the cytosolic side of adherins junctions to mediate the interaction with the actin cytoskeleton. Alpha catenin is a homodimer in solution, but dissociates to form a 1:1 heterodimer with b-catenin in order to interact with cadherins. It also regulates morphological plasticity of synapses and cerebellar and hippocampal lamination during development. Alpha catenin also functions in the control of the startle modulation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Exclusion from spheroid formation identifies loss of essential cell-cell adhesion molecules in colon cancer cells.

Scientific reports

Stadler M,Scherzer M,Walter S,Holzner S,Pudelko K,Riedl A,Unger C,Kramer N,Weil B,Neesen J,Hengstschläger M,Dolznig H

MA1-2000 was used in Western Blotting to identify molecules responsible for subpopulations of non-spheroid forming (NSF) colon cancer cell lines with distinct phenotypes during in vitro spheroid formation.

Thu Jan 18 00:00:00 EST 2018

Leptospira interrogans causes quantitative and morphological disturbances in adherens junctions and other biological groups of proteins in human endothelial cells.

PLoS neglected tropical diseases

Sato H,Coburn J

MA1-2000 was used in Immunocytochemistry-immunoflourescence to elucidate the pathogenic mechanisms of Leptospira interrogans infection.

Sat Jul 01 00:00:00 EDT 2017

Integration of left-right Pitx2 transcription and Wnt signaling drives asymmetric gut morphogenesis via Daam2.

Developmental cell

Welsh IC,Thomsen M,Gludish DW,Alfonso-Parra C,Bai Y,Martin JF,Kurpios NA

MA1-2000 was used in immunoprecipitation to study the role of Daam2 in the mechanism by which Pitx2 and Wnt participate in asymmetric gut morphogenesis

Mon Sep 30 00:00:00 EDT 2013

Characterization of cadherin-24, a novel alternatively spliced type II cadherin.

The Journal of biological chemistry

Katafiasz BJ,Nieman MT,Wheelock MJ,Johnson KR

MA1-2000 was used in western blot to investigate the functional and structural properties of cadherin 24 and its alternative splicing variant

Fri Jul 25 00:00:00 EDT 2003

Mechanism of extracellular domain-deleted dominant negative cadherins.

Journal of cell science

Nieman MT,Kim JB,Johnson KR,Wheelock MJ

MA1-2000 was used in western blot to investigate the functions of extracellular domain-deleted dominant-negative cadherin in cells

Sat May 01 00:00:00 EDT 1999

Expression of N-cadherin by human squamous carcinoma cells induces a scattered fibroblastic phenotype with disrupted cell-cell adhesion.

The Journal of cell biology

Islam S,Carey TE,Wolf GT,Wheelock MJ,Johnson KR

MA1-2000 was used in western blot to investigate the importance of N-cadherin in cell-cell adhesion

Sun Dec 01 00:00:00 EST 1996

Identification of plakoglobin domains required for association with N-cadherin and alpha-catenin.

The Journal of biological chemistry

Sacco PA,McGranahan TM,Wheelock MJ,Johnson KR

MA1-2000 was used in western blot to investigate the interaction of plakoglobin with N-cadherin and alpha catenin

Fri Aug 25 00:00:00 EDT 1995

生物信息学

蛋白别名: Alpha E-catenin; Alpha N-catenin; alpha-catenin; Alpha-catenin-related protein; alpha-E-catenin; alpha-N-catenin; alphaE-catenin; alphaN-catenin; Cadherin-associated protein; cadherin-associated protein, related; cancer/testis antigen 114; catenin; catenin (cadherin-associated protein), alpha 1, 102kDa; catenin (cadherin-associated protein), alpha 2; Catenin alpha-1; Catenin alpha-2; Catenin alpha1; Catenin alpha2; Catenin-alpha1; Catenin-alpha2; FLJ36832; FLJ52416; HGNC:2509; NY-REN-13 antigen; Renal carcinoma antigen NY-REN-13

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基因别名: CAP-R; CAP102; CAPR; CT114; CTNNA1; CTNNA2; CTNR; MDPT2

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UniProt ID: (Human) P35221, (Human) P26232

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Entrez Gene ID: (Human) 1495, (Human) 1496

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