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alpha-ENaC Antibody (MA5-45360) in IHC (P)
Immunohistochemistry analysis using Kidney. Fixation involved paraffin-embedded formalin-fixed treatment. Samples were incubated with ENaC alpha monoclonal antibody (Product # MA5-45360) at 1:100, followed by Goat Anti-Mouse ATTO 488 (green). Localization: Intercalated cells. Aquaporin 2 antibody staining in red.
产品信息
MA5-45360
产品规格
种属反应
Mouse,
Rat
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
14E10
抗原
Synthetic peptide from the N-terminal of Rat ENaC alpha (aa. 46-68)
偶联物
激发/发射光谱
482/675 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
47.35mM phosphate/2.15mM MES, pH 7.4, with 2mM EDTA, 0.17066µM Pentachlorophenol, 0.8mM ethanol
内含物
no preservative
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2931814
产品详细信息
A 1:1,000 dilution of MA5-45360 was sufficient for detection of ENaC alpha in 15 µg of Mouse whole kidney lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 85kDa.
靶标信息
Epithelial sodium channels are amiloride-sensitive members of the degenerin/epithelial sodium channel (Deg/ENaC) superfamily of ion channels. Members of this superfamily of ion channels share organizational similarity in that they all possess two short intracellular amino and carboxyl termini, two short membrane spanning segments, and a large extracellular loop with a conserved cysteine-rich region. There are three homologous isoforms of the ENaC (alpha, beta, and gamma) protein. ENaC in the kidney, lung, and colon plays an essential role in trans-epithelial sodium and fluid balance. ENaC also mediates aldosterone-dependent sodium reabsorption in the distal nephron of the kidney, thus regulating blood pressure. ENaC is thought to be regulated, in part, through association with the cystic fibrosis transmembrane conductance regulator (CFTR) chloride ion channel. Gain-of-function mutations in beta- or gamma-ENaC can cause severe arterial hypertension (Liddel’s syndrome) and loss-of-function mutations in alpha- or beta-ENaC causes pseudohypoaldosteronism (PHA-1).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Alpha-ENaC; Alpha-NaCH; amiloride-sensitive epithelial sodium channel; Amiloride-sensitive sodium channel subunit alpha; ENaC alpha; Epithelial Na(+) channel subunit alpha; epithelial sodium channel alpha subunit; FLJ21883; Nonvoltage-gated sodium channel 1 subunit alpha; SCNEA; sodium channel, nonvoltage-gated 1 alpha; sodium channel, nonvoltage-gated, type I, alpha; sodium channel, nonvoltage-gated, type I, alpha polypeptide
基因别名: ENaC; mENaC; Renac; SCNEA; Scnn1; Scnn1a
UniProt ID: (Rat) P37089, (Mouse) Q61180
Entrez Gene ID: (Rat) 25122, (Mouse) 20276
