Invitrogen

alpha Internexin Monoclonal Antibody (2E3)

alpha Internexin Antibody in Immunocytochemistry (ICC/IF)

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alpha Internexin Antibody (MA3-16726) in ICC/IF

Immunocytochemistry analysis of alpha Internexin in Hippocampal neurons in tissue culture. Samples were incubated in alpha Internexin monoclonal antibody (Product # MA3-16726). Neurofilament alpha internexin (red), using alpha-Internexin Antibody and GFAP antibody (green).

alpha Internexin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

alpha Internexin Antibody in Western Blot (WB)

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产品信息

MA3-16726

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:10,000

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:5,000

查看3篇已发表文章 3篇已发表文章

免疫细胞化学 (ICC/IF)

1:5,000

产品规格

种属反应

Bovine, Human, Mouse, Pig, Rat

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

2E3

抗原

Purified recombinant rat a-internexin.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS with 50% glycerol

内含物

5mM sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_568590

产品详细信息

The epitope recognized by the 2E3 clone is in the C-terminal non-helical extension of the protein and is unusually resistant to aldehyde fixation, so this antibody is ideal for studies of formalin-fixed paraffin-embedded histological sections.

靶标信息

alpha-Internexin is a Class IV intermediate filament subunit expressed very early in neuronal development. It is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. Many classes of mature neurons contain a-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons a-internexin is the only neurofilament subunit expressed. Antibodies to internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of a-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage. The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

TBK1 Mutation Spectrum in an Extended European Patient Cohort with Frontotemporal Dementia and Amyotrophic Lateral Sclerosis.

Human mutation

van der Zee J,Gijselinck I,Van Mossevelde S,Perrone F,Dillen L,Heeman B,Bäumer V,Engelborghs S,De Bleecker J,Baets J,Gelpi E,Rojas-García R,Clarimón J,Lleó A,Diehl-Schmid J,Alexopoulos P,Perneczky R,Synofzik M,Just J,Schöls L,Graff C,Thonberg H,Borroni B,Padovani A,Jordanova A,Sarafov S,Tournev I,de Mendonça A,Miltenberger-Miltényi G,Simões do Couto F,Ramirez A,Jessen F,Heneka MT,Gómez-Tortosa E,Danek A,Cras P,Vandenberghe R,De Jonghe P,De Deyn PP,Sleegers K,Cruts M,Van Broeckhoven C,Goeman J,Nuytten D,Smets K,Robberecht W,Damme PV,Bleecker J,Santens P,Dermaut B,Versijpt J,Michotte A,Ivanoiu A,Deryck O,Bergmans B,Delbeck J,Bruyland M,Willems C,Salmon E,Pastor P,Ortega-Cubero S,Benussi L,Ghidoni R,Binetti G,Hernández I,Boada M,Ruiz A,Sorbi S,Nacmias B,Bagnoli S,Sorbi S,Sanchez-Valle R,Llado A,Santana I,Rosário Almeida M,Frisoni GB,Maetzler W,Matej R,Fraidakis MJ,Kovacs GG,Fabrizi GM,Testi S

MA316726 was used in immunohistochemistry - paraffin section to examine the mutation spectrum of the TANK-Binding Kinase 1 gene and its associated phenotypic spectrum in patients with FTD, ALS or FTD plus ALS

Wed Mar 01 00:00:00 EST 2017

Neuropathological criteria of anti-IgLON5-related tauopathy.

Acta neuropathologica

Gelpi E,Höftberger R,Graus F,Ling H,Holton JL,Dawson T,Popovic M,Pretnar-Oblak J,Högl B,Schmutzhard E,Poewe W,Ricken G,Santamaria J,Dalmau J,Budka H,Revesz T,Kovacs GG

MA316726 was used in immunohistochemistry - paraffin section to detail the neuropathological features of the anti-IgLON5 syndrome in order to diagnose patients

Sat Oct 01 00:00:00 EDT 2016

An autopsy case of neuronal intermediate filament inclusion disease with regard to immunophenotypic and topographical analysis of the neuronal inclusions.

Neuropathology : official journal of the Japanese Society of Neuropathology

Inoue K,Fujimura H,Ueda K,Matsumura T,Itoh K,Sakoda S

MA3-16726 was used in immunohistochemistry - paraffin section to report an autopsy case of neuronal intermediate filament inclusion disease.

Tue Dec 01 00:00:00 EST 2015

Cytoplasmic non-epithelial mucin accumulation associated with CD44 in an astrocytic tumor with signet ring features.

Brain tumor pathology

Okabe H,Nagata A,Katsura K,Ishida M,Osaka Y,Tenjin H

MA3-16726 was used in immunohistochemistry to report on a case of an unusual astrocytic tumor rich in signet ring cells

Tue Apr 01 00:00:00 EDT 2014

Gliosarcoma with ependymal and PNET-like differentiation.

Clinical neuropathology

Shintaku M,Yoneda H,Hirato J,Nagaishi M,Okabe H

MA3-16726 was used in immunohistochemistry to characterize a rare case of gliosarcoma

Thu Mar 13 00:00:00 EDT 2014

生物信息学

蛋白别名: 66 kDa neurofilament protein; Alpha-internexin; Alpha-Inx; FLJ18662; FLJ57501; internexin neuronal intermediate filament protein, alpha; internexin, alpha; MGC12702; Neuro; Neurofilament 5; neurofilament 5 (66kD); Neurofilament 66; neurofilament-66; neurofilament-66, tax-binding protein; NF-66

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基因别名: AV028420; INA; Inexa; Intlaa; NEF5; NF-66; NF66; TXBP-1

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UniProt ID: (Human) Q16352, (Bovine) Q08DH7, (Mouse) P46660