Invitrogen

alpha Synuclein Recombinant Mouse Monoclonal Antibody (10E5)

查看其他66个alpha Synuclein抗体

抗体检测数据 (4)

alpha Synuclein Antibody in Immunocytochemistry (ICC/IF)

alpha Synuclein Antibody in Immunocytochemistry (ICC/IF)

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alpha Synuclein Antibody (MA5-50223) in ICC/IF

Immunocytochemistry analysis of SH-SY5Y cell with alpha Synuclein monoclonal antibody (Product # MA5-50223) at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

alpha Synuclein Antibody in Immunocytochemistry (ICC/IF)

alpha Synuclein Antibody in Immunohistochemistry (Paraffin) (IHC (P))

alpha Synuclein Antibody in Immunohistochemistry (Paraffin) (IHC (P))

alpha Synuclein Antibody in Flow Cytometry (Flow)

Product Image Gallery

alpha Synuclein Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of SH-SY5Y cell with alpha Synuclein monoclonal antibody (Product # MA5-50223) at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

alpha Synuclein Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis with a alpha Synuclein monoclonal antibody (Product # MA5-50223) using a dilution of 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

alpha Synuclein Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis with a alpha Synuclein monoclonal antibody (Product # MA5-50223) using a dilution of 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

alpha Synuclein Antibody in Flow Cytometry (Flow) — Flow cytometry analysis overlay peak curve showing SH-SY5Y cells stained with alpha Synuclein monoclonal antibody (Product # MA5-50223) (red line) at 1x10^6 cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1x10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

产品信息

MA5-50223

应用

建议稀释比

已发表文章

免疫组化（石蜡） (IHC (P))

1:20-1:200

-

免疫细胞化学 (ICC/IF)

1:20-1:200

-

流式细胞分析 (Flow)

1:20-1:200

-

酶联免疫吸附实验 (ELISA)

Assay-dependent

-

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG2a

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

10E5

抗原

Recombinant Human SNCA protein

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.03% Proclin 300

保存条件

-20°C or -80°C if preferred

运输条件

Wet ice

RRID

AB_3092452

靶标信息

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. These proteins are abundant in the brain and play a role in regulating synaptic transmission and membrane trafficking. Alpha- and beta-synuclein have been shown to selectively inhibit phospholipase D2, suggesting that they may also be involved in signaling pathways. Defects in the SNCA gene, which encodes alpha-synuclein, have been implicated in the pathogenesis of Parkinson's disease. Additionally, alpha-synuclein peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Multiple alternatively spliced transcripts encoding different isoforms have been identified for this gene. Diseases associated with SNCA include Parkinson Disease 1, Autosomal Dominant and Dementia, Lewy Body.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Alpha synuclein; Alpha-synuclein; NACP; non A-beta component of AD amyloid; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; synuclein alpha-140; synuclein, alpha (non A4 component of amyloid precursor)

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基因别名: NACP; PARK1; PARK4; PD1; SNCA

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UniProt ID: (Human) P37840

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Entrez Gene ID: (Human) 6622

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magnesium ion binding fatty acid binding copper ion binding calcium ion binding protein binding phospholipid binding microtubule binding ferrous iron binding zinc ion binding oxidoreductase activity kinesin binding protein domain specific binding histone binding identical protein binding alpha-tubulin binding cysteine-type endopeptidase inhibitor activity involved in apoptotic process phospholipase binding transcription regulatory region DNA binding dynein binding protein N-terminus binding tau protein binding beta-tubulin binding phosphoprotein binding phospholipase D inhibitor activity membrane trafficking regulatory protein

参与通路

negative regulation of transcription from RNA polymerase II promoter microglial cell activation positive regulation of receptor recycling negative regulation of protein phosphorylation positive regulation of neurotransmitter secretion fatty acid metabolic process neutral lipid metabolic process phospholipid metabolic process apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process mitochondrial membrane organization aging adult locomotory behavior response to iron(II) ion regulation of phospholipase activity negative regulation of platelet-derived growth factor receptor signaling pathway regulation of glutamate secretion regulation of dopamine secretion negative regulation of microtubule polymerization receptor internalization protein destabilization response to magnesium ion negative regulation of transporter activity response to lipopolysaccharide negative regulation of monooxygenase activity positive regulation of peptidyl-serine phosphorylation response to interferon-gamma cellular response to oxidative stress negative regulation of histone acetylation regulation of locomotion dopamine biosynthetic process response to drug mitochondrial ATP synthesis coupled electron transport regulation of macrophage activation positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process extracellular fibril organization negative regulation of neuron apoptotic process cellular protein metabolic process cellular response to fibroblast growth factor stimulus positive regulation of endocytosis negative regulation of exocytosis negative regulation of dopamine metabolic process behavioral response to cocaine regulation of long-term neuronal synaptic plasticity synaptic vesicle endocytosis synapse organization regulation of acyl-CoA biosynthetic process positive regulation of release of sequestered calcium ion into cytosol dopamine uptake involved in synaptic transmission negative regulation of dopamine uptake involved in synaptic transmission negative regulation of serotonin uptake negative regulation of norepinephrine uptake calcium ion homeostasis oxidation-reduction process excitatory postsynaptic potential long-term synaptic potentiation positive regulation of inositol phosphate biosynthetic process negative regulation of thrombin receptor signaling pathway response to interleukin-1 cellular response to copper ion cellular response to epinephrine stimulus positive regulation of protein serine/threonine kinase activity negative regulation of neuron death negative regulation of mitochondrial electron transport, NADH to ubiquinone positive regulation of glutathione peroxidase activity positive regulation of hydrogen peroxide catabolic process regulation of synaptic vesicle recycling regulation of reactive oxygen species biosynthetic process negative regulation of chaperone-mediated autophagy