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Invitrogen
beta-3 Tubulin Monoclonal Antibody (2G10-TB3), Alexa Fluor™ 488, eBioscience™
高级验证
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
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beta-3 Tubulin Antibody (53-4510-82) in ICC/IF
Knockout of beta-3 Tubulin (TUBB3) was achieved by CRISPR-Cas9 genome editing. Immunofluorescence analysis was performed on wild type U-87 MG cells (panel a,d), U-87 MG Cas9 cells (panels b,e) and U-87 MG beta-3 Tubulin KO cells (panel c,f). Cells were fixed, permeabilized, and labelled with beta-3 Tubulin Monoclonal Antibody, Alexa Fluor® 488 (Product # 53-4510-82) (10 µg/mL). Nuclei (blue) were stained us... View More
产品信息
53-4510-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine,
Human,
Mouse,
Rat
已发表种属
Human
宿主/亚型
Mouse
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
2G10-TB3
偶联物
激发/发射光谱
499/520 nm
查看光谱
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2574421
产品详细信息
Description: The 2G10 monoclonal antibody recognizes beta tubulin class III, a member of the large family of tubulins that are components of the microtubules of the cytoskeletal network. Beta tubulin class III expression is exclusively found in neuronal cells of adult mammals. Although expression has been mainly found in neuronal cells, during fetal development, transient expression has been documented in both glial and neuronal precursors localized to the subventricular zone. Identification of beta tubulin class III expression is useful as a structural marker for developing and mature neural cells. Given the high degree of conservation of this protein across species, the 2G10 clone is expected to recognize human, mouse, rat, and bovine beta tubulin class III.
Applications Reported: This 2G10-TB3 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry.
Applications Tested: This 2G10-TB3 antibody has been tested by immunocytochemistry of formaldehyde-fixed and permeabilized cells and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
The betaIII-tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: beta 3 tubulin; beta tubulin 3; betaIII-tubulin; class III beta-tubulin; MC1R; Neuron specific beta III Tubulin; Neuron-specific class III beta-tubulin; TUBB; tubulin beta; Tubulin beta-3; Tubulin beta-3 chain; Tubulin beta-4; Tubulin beta-4 chain; Tubulin beta-III; tubulin, beta 3 class III; tubulin, beta, 3; tubulin, beta, 4
基因别名: 3200002H15Rik; beta-4; CDCBM; CDCBM1; CFEOM3; CFEOM3A; FEOM3; M(beta)3; M(beta)6; TUBB3; TUBB4
UniProt ID: (Bovine) Q2T9S0, (Human) Q13509, (Mouse) Q9ERD7, (Rat) Q4QRB4
Entrez Gene ID: (Bovine) 768070, (Human) 10381, (Mouse) 22152, (Rat) 246118
