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beta Actin Antibody (PA5-85291) in ICC/IF
beta Actin Polyclonal Antibody detects beta Actin protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in 0.5% Triton X-100 for 1 min, then ice-cold methanol for 5 min. Green: beta Actin protein stained by beta Actin Polyclonal Antibody (Product # PA5-85291) diluted at 1:500. Blue: Hoechst 33342 staining.
产品信息
PA5-85291
应用
建议稀释比
已发表文章
产品规格
种属反应
Fruit fly,
Human,
Mouse,
Rat
已发表种属
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein encompassing a sequence within the center region of human beta Actin.
偶联物
形式
Liquid
浓度
0.26 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol, 1% BSA
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2792434
产品详细信息
Keep as concentrated solution.
Predicted reactivity: Mouse (100%), Rat (100%), Japanese Medaka (98%), Xenopus laevis (99%), Dog (100%), Rabbit (99%), Chicken (100%), Sheep (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%), Guinea pig (100%).
Positive Control: 293T, A431, HeLa, HepG2, Neuro2A, CD830, NIH-3T3, Raw264.7, C2C12, mouse liver, rat liver, PC-12, Rat2, drosophila.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: actin, beta; Actin, cytoplasmic 1; beta cytoskeletal actin; Beta-actin; cytoplasmic beta-actin; PS1TP5-binding protein 1
基因别名: ACTB; Actx; beta-actin; BRWS1; E430023M04Rik; PS1TP5BP1
UniProt ID: (Human) P60709, (Rat) P60711, (Mouse) P60710
Entrez Gene ID: (Human) 60, (Rat) 81822, (Mouse) 11461
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
structural constituent of cytoskeleton
protein binding
ATP binding
kinesin binding
Tat protein binding
nucleosomal DNA binding
identical protein binding
nitric-oxide synthase binding
protein kinase binding
actin and actin related protein
actin or actin-binding cytoskeletal protein
cytoskeletal protein
retina homeostasis
movement of cell or subcellular component
cytoskeleton organization
substantia nigra development
cell junction assembly
Fc-gamma receptor signaling pathway involved in phagocytosis
ATP-dependent chromatin remodeling
positive regulation of gene expression, epigenetic
vascular endothelial growth factor receptor signaling pathway
ephrin receptor signaling pathway
platelet aggregation
axonogenesis
retina development in camera-type eye
cellular response to electrical stimulus
