Invitrogen

beta Actin Recombinant Rabbit Monoclonal Antibody (JF53-10), HRP

beta Actin Antibody in Western Blot (WB)

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beta Actin Antibody (MA5-32540) in WB

Western blot analysis of beta Actin in different lysates using a Monoclonal antibody (Product #MA5-32540) at a dilution of 1:1,000. Positive control: Lane 1: Hela, Lane 2: PC-12, Lane 3: NIH/3T3, Lane 4: zebrafish.

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产品信息

MA5-32540

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:10,000

查看4篇已发表文章 4篇已发表文章

产品规格

种属反应

Hamster, Human, Mouse, Non-human primate, Plant, Rat, Zebrafish

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JF53-10

抗原

Recombinant full length protein of Human beta Actin aa 1-375

查看免疫原

偶联物

HRP HRP HRP

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2809817

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: actin, beta; Actin, cytoplasmic 1; B-ACTZF; beta cytoskeletal actin; Beta-actin; Beta-actin-1; cytoplasmic beta-actin; PS1TP5-binding protein 1

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基因别名: ACTB; actba; Actx; B-ACTZF; bact; bactin1; bactzf; Beta-actin; BRWS1; E430023M04Rik; PS1TP5BP1; wu:fd18f05