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beta Amylase Antibody (200-4647-0100) in DB
Dot Blot results of Rabbit Anti-Beta Amylase Biotin Conjugated. Antigen: Beta Amylase. Blot loaded at 3 fold dilution: 1. 100ng, 2. 33.3ng, 3. 11.1ng, 4. 3.70ng, 5. 1.23ng. Blocking: MB-070 Buffer for 30 minutes at RT. Primary Antibody: Rabbit Anti-Beta Amylase Biotin 1µg/mL for 1hr at RT. Secondary Antibody: Goat anti-Rabbit HRP (p/n 611-103-122) at 1:70,000 for 30min at RT. Imaging System ChemiDoc, Filter used: Chemi.
产品信息
200-4647-0100
应用
建议稀释比
已发表文章
产品规格
种属反应
Plant
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Beta Amylase [Sweet Potato]
偶联物
形式
Lyophilized
浓度
1 mg/mL
纯化类型
Ion-exchange chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10mg/mL BSA
内含物
0.01% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Beta Amylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified b-Amylase [Sweet Potato]. Cross reactivity against b-Amylase from other sources may occur but have not been specifically determined.
靶标信息
Beta Amylase is an enzyme found in the seeds of higher plants and in sweet potatoes. It hydrolyses 1,4 alpha D glucosidic linkages in polysaccharides to remove successive maltose units from the non reducing ends of the chains. Used in the structural analysis of starch and glycogen.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ß-amylase
