Invitrogen

beta Tubulin Polyclonal Antibody

beta Tubulin Antibody in Immunocytochemistry (ICC/IF)

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beta Tubulin Antibody (PA1-16947) in ICC/IF

Immunofluorescence analysis of beta Tubulin was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Beta Tubulin Rabbit Polyclonal Antibody (Product # PA1-16947) at 2 µg/mL in 0.1% BSA and incubated for 3 ho... View More

beta Tubulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

beta Tubulin Antibody in Western Blot (WB)

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产品信息

PA1-16947

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看6篇已发表文章 6篇已发表文章

免疫组化（石蜡） (IHC (P))

1:1,000-1:2,000

免疫细胞化学 (ICC/IF)

1:500-1:1,000

其他PubMed (Misc)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Bovine, C. elegans, Chicken, Hamster, Human, Insect, Invertebrate, Mouse, Non-human primate, Pig, Rat, Xenopus, Zebrafish

已发表种属

Human, Mouse, Rhesus monkey

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide between amino acids 1-100 of the human Beta-Tubulin protein.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_795659

产品详细信息

Suggested positive control: antigen standard for TUBB (transient overexpression lysate), Hela whole cell extract.

靶标信息

Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance and maintenance. Mutations in the beta tubulin gene are the cause of congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues. Glutamate residues at the C-terminus of beta III tubulin can be glutamylated. The precise function of such modifications is unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression. Ser-172 phosphorylation inhibits tubulin incorporation into microtubules.Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55 kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. Because beta-tubulin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

E4orf1 Suppresses E1B-Deleted Adenovirus Vaccine-Induced Immune Responses.

Vaccines

Sangare K,Helmold Hait S,Moore M,Hogge C,Hoang T,Rahman MA,Venzon DJ,LaBranche C,Montefiori D,Robert-Guroff M,Thomas MA

PA1-16947 was used in Western Blot to show that cells infected with an E1B55K and E4orf1-deleted (ΔE41) Ad exhibited reduced levels of phosphorylated Akt (Ser473 and Thr308)) and expressed different intrinsic innate immune cytokines from those induced in cells infected with an E4orf1-containing, ΔE1B parental Ad that exhibited elevated levels of phosphorylated Akt.

Tue Feb 15 00:00:00 EST 2022

E4orf1 Suppresses E1B-Deleted Adenovirus Vaccine-Induced Immune Responses.

Vaccines

Sangare K,Helmold Hait S,Moore M,Hogge C,Hoang T,Rahman MA,Venzon DJ,LaBranche C,Montefiori D,Robert-Guroff M,Thomas MA

Tue Feb 15 00:00:00 EST 2022

Leishmania donovani parasite requires Atg8 protein for infectivity and survival under stress.

Cell death & disease

Giri S,Shaha C

PA1-16947 was used in Western Blotting to show that Atg8 plays a role in sustaining Leishmania parasite survival during its life cycle and stress exposure.

Thu Oct 24 00:00:00 EDT 2019

Persistent disruption of lateral junctional complexes and actin cytoskeleton in parotid salivary glands following radiation treatment.

American journal of physiology. Regulatory, integrative and comparative physiology

Wong WY,Pier M,Limesand KH

PA1-16947 was used in Western Blotting to evaluate changes in adherens junctions and cytoskeletal regulators in an injury model where mice were irradiated with 5 Gy and a restoration model where mice injected postradiation with insulin-like growth factor 1 (IGF1) are capable of restoring salivary function.

Mon Oct 01 00:00:00 EDT 2018

The Role of Sonic Hedgehog in the Specification of Human Cortical Progenitors In Vitro.

Cerebral cortex (New York, N.Y. : 1991)

Radonjić NV,Memi F,Ortega JA,Glidden N,Zhan H,Zecevic N

PA1-16947 was used in western blot to study the relation between human cortical formation and impaired sonic hedgehog signaling

Fri Jan 01 00:00:00 EST 2016

Synthesis of the Proposed Structure of Damaurone D and Evaluation of Its Anti-inflammatory Activity.

Chemical & pharmaceutical bulletin

Han YT,Wang Z,Bae EJ

PA1-16947 was used in western blot to describe a method to synthesize damaurone D and test its immunomodulatory activity

Tue May 17 00:00:00 EDT 2016

The new 4-O-methylhonokiol analog GS12021 inhibits inflammation and macrophage chemotaxis: role of AMP-activated protein kinase α activation.

PloS one

Kim S,Ka SO,Lee Y,Park BH,Fei X,Jung JK,Seo SY,Bae EJ

PA1-16947 was used in western blot to determine if methylhonokiol analogs inhibit the expression of inflammatory genes in macrophages and adipocytes

Wed Jan 13 00:00:00 EST 2016

Inhibition of adipocyte inflammation and macrophage chemotaxis by butein.

European journal of pharmacology

Wang Z,Lee Y,Eun JS,Bae EJ

PA1-16947 was used in western blot to study the mechanisms underlying the inhibitory effects of butein on adipocyte inflammation and macrophage chemotaxis

Fri Sep 05 00:00:00 EDT 2014

生物信息学

蛋白别名: beta 5-tubulin; beta Ib tubulin; beta tubulin; beta tubulin 1; beta-4 tubulin; beta-4-tubulin; beta-5 tubulin; beta-tubulin; Beta-tubulin class-I; Beta-tubulin class-II; beta-tubulin class-III; Beta-tubulin class-V; Beta-tubulin class-VI; Beta-tubulin isotype I; beta-tubulin T beta15 (aa 1-445); beta5-tubulin; betaIII-tubulin; c(beta)7 tubulin; class I beta tubulin; Class I beta-tubulin; class II beta-tubulin isotype; class IIa beta-tubulin; class IIb beta-tubulin; class III beta-tubulin; class V beta tubulin; class V beta-tubulin; dJ40E16.7; dystonia 4, torsion (autosomal dominant); HSA10p15 beta-tubulin 4Q; M40 antibody; Neuron-specific class III beta-tubulin; T beta-15; TUBB; tubb beta-5; tubb2a {ECO:0000250|UniProtKB:Q7TMM9}; Tubulin 5 beta; tubulin beta; Tubulin beta 4'; Tubulin beta 8 class VIII; Tubulin beta chain; Tubulin beta class IIa; Tubulin beta class V; tubulin beta MGC4083; tubulin beta-1; Tubulin beta-1 chain; Tubulin beta-2 chain; Tubulin beta-2A chain; Tubulin beta-2B chain; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-4A chain; Tubulin beta-5 chain; Tubulin beta-6 chain; Tubulin beta-7 chain; Tubulin beta-8 chain; Tubulin beta-III; tubulin, beta 1 class VI; tubulin, beta 2; tubulin, beta 2A; tubulin, beta 2A class IIa; tubulin, beta 2B class IIb; tubulin, beta 3; tubulin, beta 3 class III; tubulin, beta 4; tubulin, beta 4 class IVa; tubulin, beta 4A class IVa; tubulin, beta 5; tubulin, beta 6 class V; tubulin, beta class I; tubulin, beta family 1; tubulin, beta polypeptide; tubulin, beta polypeptide 2; tubulin, beta polypeptide paralog; tubulin, beta, 3; tubulin, beta, 4; tubulin, beta, 5; unnamed protein product; zbetaI

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基因别名: 2310057H16Rik; 2410129E14Rik; 2810484G07Rik; 3200002H15Rik; AA408537; AI325297; AI596182; B130022C14Rik; bA506K6.1; bA631M21.2; BB220206; beta-4; beta-5; brdp; CDCBM; CDCBM1; CDCBM5; CDCBM6; CFEOM3; CFEOM3A; CSCSC1; DYT4; fb40f09; FEOM3; HsT1601; I79_000194; I79_012097; M(beta)1; M(beta)2; M(beta)3; M(beta)4; M(beta)5; M(beta)6; M40; OK/SW-cl.56; OOMD; OOMD2; PMGYSA; RGD1305887; RGD1309427; si:ch211-122i22.4; si:ch211-9a18.2; TUBB; TUBB-5; TUBB1; TUBB2; TUBB2A; TUBB2B; TUBB3; TUBB4; TUBB4A; TUBB5; TUBB6; TUBB8; wu:fb40f09; XELAEV_18039222mg; XLOT; zgc:112515; zgc:123194