GenScript

beta-tubulin Monoclonal Antibody (2G7D4)

beta-tubulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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beta-tubulin Antibody (A01717-40) in IHC (P)

Immunohistochemistry analysis of human colon tissue slide (Paraffin embedded) using beta-tubulin Antibody (2G7D4), mAb, Mouse (GenScript, Cat. No. A01717, 15 µg/mL).

beta-tubulin Antibody in Western Blot (WB)

beta-tubulin Antibody in Flow Cytometry (Flow)

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产品信息

A01717-40

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.2-1 µg/mL

免疫组化（石蜡） (IHC (P))

10-20 µg/mL

流式细胞分析 (Flow)

1 µg 1 x 10^6 cells

其他PubMed (Misc)

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产品规格

种属反应

Bovine, Chicken, Fish, Goat, Human, Mouse, Non-human primate, Pig, Rabbit, Rat

已发表种属

Not Applicable

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

2G7D4

抗原

purified human recombinant full-length beta-tubulin protein

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.02% sodium azide

保存条件

-20°C or -80°C if preferred

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. It is a dimer of alpha (a) and beta (ß) chains. ß-tubulin is ubiquitously expressed with highest levels in spleen, thymus and immature brain and it has a highly acidic C-terminal region which may bind cations such as calcium. This antibody can be used as a loading control on Western blots.GenScript ß-tubulin Antibody (2G7D4), mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with purified human recombinant full-length ß-tubulin protein.

靶标信息

Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance and maintenance. Mutations in the beta tubulin gene are the cause of congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues. Glutamate residues at the C-terminus of beta III tubulin can be glutamylated. The precise function of such modifications is unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression. Ser-172 phosphorylation inhibits tubulin incorporation into microtubules.Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55 kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. Because beta-tubulin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Cyclic cis-Locked Phospho-Dipeptides Reduce Entry of AβPP into Amyloidogenic Processing Pathway.

Journal of Alzheimer's disease : JAD

Fisher CL,Resnick RJ,De S,Acevedo LA,Lu KP,Schroeder FC,Nicholson LK

The cis/trans isomerization of X-Pro peptide bonds in proteins in some instances acts as a molecular switch in biological pathways. Our prior work suggests that the cis isomer of the phospho-Thr668-Pro669 motif located in the cytoplasmic domain of the amyloid-β protein precursor (AβPP) is correlated with an increase in amyloidogenic processing of AβPP and production of amyloid-beta (Aβ) the neurotoxic peptide fragment in Alzheimer's disease (AD). We designed a 100% cis-locked

Thu Feb 15 00:00:00 EST 2018

Acyl-coenzyme A:cholesterol acyltransferase 1 - significance of single-nucleotide polymorphism at residue 526 and the role of Pro347 near the fifth transmembrane domain.

The FEBS journal

Huang LH,Nishi K,Li S,Ho T,Dong R,Chang CC,Chang TY

Acyl-Coenzyme A:cholesterol Acyltransferases (ACATs), members of the membrane bound O-acyltransferase (MBOAT) family, catalyze the conversion of cholesterol to cholesteryl esters. Mammals contain two isoenzymes ACAT1 and ACAT2. Both enzymes are drug targets for treating human diseases. ACAT1 is present ubiquitously in various cell types. It contains 9 transmembrane domains (TMDs) with the active site H460 locating within TMD #7, and another active site N421 locating within the 4th large cytoplas

Tue Apr 01 00:00:00 EDT 2014

生物信息学

蛋白别名: beta 5-tubulin; beta Ib tubulin; beta tubulin 1; beta-4 tubulin; beta-4-tubulin; beta-5 tubulin; beta-tubulin; Beta-tubulin class-I; Beta-tubulin class-II; beta-tubulin class-III; Beta-tubulin class-V; Beta-tubulin class-VI; beta-tubulin T beta15 (aa 1-445); betaIII-tubulin; c(beta)7 tubulin; class II beta-tubulin isotype; class IIa beta-tubulin; class IIb beta-tubulin; class III beta-tubulin; class V beta-tubulin; dJ40E16.7; dystonia 4, torsion (autosomal dominant); HSA10p15 beta-tubulin 4Q; M40 antibody; Neuron-specific class III beta-tubulin; T beta-15; TUBB; tubb2a {ECO:0000250|UniProtKB:Q7TMM9}; Tubulin 5 beta; tubulin beta; Tubulin beta 4'; Tubulin beta 8 class VIII; Tubulin beta chain; Tubulin beta class IIa; Tubulin beta class V; tubulin beta MGC4083; Tubulin beta-1 chain; Tubulin beta-2 chain; Tubulin beta-2A chain; Tubulin beta-2B chain; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-4A chain; Tubulin beta-5 chain; Tubulin beta-6 chain; Tubulin beta-7 chain; Tubulin beta-8 chain; Tubulin beta-III; tubulin, beta 1 class VI; tubulin, beta 2; tubulin, beta 2A; tubulin, beta 2A class IIa; tubulin, beta 2B class IIb; tubulin, beta 3; tubulin, beta 3 class III; tubulin, beta 4; tubulin, beta 4 class IVa; tubulin, beta 4A class IVa; tubulin, beta 6 class V; tubulin, beta class I; tubulin, beta family 1; tubulin, beta polypeptide; tubulin, beta polypeptide 2; tubulin, beta polypeptide paralog; tubulin, beta, 3; tubulin, beta, 4; tubulin, beta, 5; unnamed protein product

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基因别名: 2310057H16Rik; 2410129E14Rik; 2810484G07Rik; 3200002H15Rik; AA408537; AI325297; AI596182; B130022C14Rik; bA506K6.1; bA631M21.2; BB220206; beta-4; beta-5; brdp; CDCBM; CDCBM1; CDCBM5; CDCBM6; CFEOM3; CFEOM3A; CSCSC1; DYT4; FEOM3; HsT1601; M(beta)1; M(beta)2; M(beta)3; M(beta)4; M(beta)5; M(beta)6; M40; OK/SW-cl.56; OOMD; OOMD2; PMGYSA; RGD1305887; RGD1309427; TUBB; TUBB-5; TUBB1; TUBB2; TUBB2A; TUBB2B; TUBB3; TUBB4; TUBB4A; TUBB5; TUBB6; TUBB8