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c-Fos Antibody (MA5-45884) in IHC
Immunohistochemistry analysis using Kidney. Samples were incubated with c-Fos monoclonal antibody (Product # MA5-45884) at 1:100 for Overnight at 4C, then 30 min at 37C, followed by Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain used was DAPI for 3 min at RT. Magnification: 20X.
产品信息
MA5-45884
产品规格
宿主/亚型
Mouse
/ IgG2
分类
Monoclonal
类型
Antibody
克隆号
4A3
抗原
Synthetic peptide from the N-terminal of Human c-Fos (aa. 280-380)
偶联物
激发/发射光谱
498/517 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
保存条件
4° C, store in dark
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
A 1:1,000 dilution of MA5-45884 was sufficient for detection of c-fos in 10 µg of serum starved HeLa cells treated with PMA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects 62 kDa band. Observed molecular weight of band is higher than predicted 41 kDa band due to post translational modifications.
靶标信息
FBJ murine osteosarcoma viral oncogene homolog is commonly known as Cellular protooncogene Fos (c-Fos). It belongs to the fos transcription factor family and is encoded by the C-FOS gene located on chromosome 14. c-Fos heterodimerizes with c-Jun to form the Activator Protein -1 (AP-1) transcription factor which regulates transcription of several genes that play an important role in cellular signal transduction, cell proliferation and differentiation. c-Fos has been shown to be phosphorylated by various stimuli including growth factors and insulin at atleast seven different sites. The best known upstream kinase for phosphorylation at Threonine 232 is ERK MAPK which regulates localization of c-Fos to the nucleus and is thus important for c-Fos induced transcriptional activity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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