Bethyl Laboratories

c-Ski Polyclonal Antibody

c-Ski Antibody in Immunoprecipitation (IP)

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c-Ski Antibody (A303-517A) in IP

Detection of human c-Ski by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-c-Ski antibody A303-517A used for IP at 6 µg/mg lysate. c-Ski was also immunoprecipitated by rabbit anti-c-Ski antibody A303-518A, which recognizes a downstream epitope. For blotting immunoprecipitated c-Ski, A303-518A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

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产品信息

A303-517A

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

免疫沉淀 (IP)

2-10 µg/mg lysate

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Region between residue 450 and 500 of human v-Ski Sarcoma Viral Oncogene Homolog.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

phosphate/tris citrate, pH 7-8

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Wet ice

产品详细信息

The recommended shelf life for this product is 1 year from date of receipt.

靶标信息

TGF-beta is a ubiquitously expressed cytokine that signals through the Smad protein family to regulate numerous cellular processes such as embryonic development and tumorigenesis. This signaling is negatively regulated by Ski, the mammalian homolog of the transforming protein of an avian retrovirus that induces oncogenic transformation of chicken embryo cells, and the related protein SnoN. Ski functions by binding to the Smad proteins and preventing their phosphorylation, thereby inhibiting their ability to bind DNA and activate the transcription of downstream genes. Ski will also bind to the Smad proteins specific to bone morphogenic proteins (BMPs) and block BMP signaling in mammalian cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。