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eEF1G Antibody (A300-735A) in WB
Detection of human and mouse eEF1G by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-eEF1G antibody (Product # A300-735A) used for WB at 0.04 µg/mL (A) and 1 µg/mL (B). eEF1G was immunoprecipitated using rabbit anti-eEF1G antibody (Product # A300-734A) at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds... View More
产品信息
A300-735A
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Region between residue 400 and the C-terminus (residue 437) of human Eukaryotic Translation Elongation Factor 1 Gamma.
偶联物
形式
Liquid
浓度
0.20 mg/mL
纯化类型
Antigen affinity chromatography
保存液
TBS, pH 7.0 to 8.0, with 0.1% BSA
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Wet ice
产品详细信息
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Bovine, Horse and Pig
靶标信息
EF-1 (elongation factor-1) is a multi-protein complex that is responsible for the delivery of aminoacyl-tRNAs to the ribosome. EF-1 gamma (elongation factor- 1 gamma), also known as EEF1G or GIG35, is a 437 amino acid subunit of the EF-1 complex. Expressed in stomach, pancreas, brain, lung, kidney, intestine, liver and spleen, EF-1 gamma contains an N-terminal Glutathione transferase domain which is thought to be involved in anchoring the complex to various cellular components. Additionally, EF-1 gamma may play a key role in the assembly of multiprotein complexes containing aminoacyl-tRNA synthetases. Increased expression of EF-1 gamma is associated with pancreatic cancer, suggesting a possible role for EF-1 gamma in the oncogenic transformation process.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: eEF-1B gamma; EF-1-gamma; Elongation factor 1-gamma; pancreatic tumor-related protein; PRO1608; translation elongation factor eEF-1 gamma chain
基因别名: 2610301D06Rik; AA407312; EEF1G; EF1G; GIG35; PRO1608
UniProt ID: (Human) P26641, (Mouse) Q9D8N0
Entrez Gene ID: (Human) 1937, (Mouse) 67160
