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Invitrogen
eIF4G Polyclonal Antibody
高级验证
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
图: 1 / 8
eIF4G Antibody (PA5-17422) in ICC/IF
Immunofluorescence analysis of eIF4G was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with eIF4G Rabbit Polyclonal Antibody(Product # PA5-17422) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product #... View More
产品信息
PA5-17422
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Non-human primate,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to a sequence of human eIF4G
偶联物
形式
Liquid
浓度
7 µg/mL
纯化类型
Antigen affinity chromatography
保存液
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_10974330
产品详细信息
It is not recommended to aliquot this antibody.
靶标信息
The protein encoded by this gene is a component of the protein complex EIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure, and recruitment of mRNA to the ribosome. Alternative splicing results in five transcript variants encoding four distinct isoforms.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DKFZp686A1451; eIF-4-gamma 1; EIF4-gamma; eucaryotic translation initiation factor 4G; Eukaryotic translation initiation factor 4 gamma 1; eukaryotic translation initiation factor 4 gamma, 1; OTTHUMP00000210577; OTTHUMP00000210586; OTTHUMP00000210587; OTTHUMP00000210588; OTTHUMP00000210595; OTTHUMP00000210602; OTTHUMP00000210603; p220
基因别名: E030015G23Rik; eIF-4-gamma 1; eIF-4G 1; EIF-4G1; EIF4F; EIF4G; EIF4G1; EIF4GI; P220; PARK18
UniProt ID: (Human) Q04637, (Mouse) Q6NZJ6
Entrez Gene ID: (Human) 1981, (Mouse) 208643, (Rat) 287986
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
nuclear-transcribed mRNA poly(A) tail shortening
behavioral fear response
translational initiation
regulation of translational initiation
viral process
regulation of mRNA stability
positive regulation of neuron differentiation
cell-cell adhesion
lung development
response to ethanol
