Invitrogen

gamma Tubulin Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1个已发表图片

3 篇参考文献

查看其他23个gamma Tubulin抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

gamma Tubulin Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 11

gamma Tubulin Antibody (PA5-34815) in ICC/IF

gamma Tubulin Polyclonal Antibody detects gamma Tubulin protein at centrosome by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: gamma Tubulin stained by gamma Tubulin Polyclonal Antibody (Product # PA5-34815) diluted at 1:1,000. Red: alpha Tubulin, stained by alpha Tubulin Polyclonal Antibody [GT114] (Product # MA5-31466) diluted at 1:500. Blue: Fluoroshield wit... View More

gamma Tubulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

gamma Tubulin Antibody in Western Blot (WB)

gamma Tubulin Antibody in Immunoprecipitation (IP)

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产品信息

PA5-34815

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫组织化学（多聚甲醛固定） (IHC (PFA))

Assay-dependent

免疫细胞化学 (ICC/IF)

1:100-1:1,000

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

1:100-1:500

其他PubMed (Misc)

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产品规格

种属反应

Human, Mouse, Rat, Zebrafish

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 1 and 184 of gamma Tubulin (Uniprot ID#P23258)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.24 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 1% BSA, 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2552167

产品详细信息

Recommended positive controls: 293T, A431, HeLa, Jurkat, Raji, NCI-H929, NIH3T3, rat kidney.

Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (98%), Xenopus laevis (99%), Dog (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

This gene encodes a protein that interacts with thyroid hormone receptors, also known as hormone-dependent transcription factors. The gene product interacts specifically with the ligand binding domain. This gene is one of several that may play a role in early-stage non-small cell lung cancer.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Phosphoproteomic analysis of neoadjuvant breast cancer suggests that increased sensitivity to paclitaxel is driven by CDK4 and filamin A.

Nature communications

Mouron S,Bueno MJ,Lluch A,Manso L,Calvo I,Cortes J,Garcia-Saenz JA,Gil-Gil M,Martinez-Janez N,Apala JV,Caleiras E,Ximénez-Embún P,Muñoz J,Gonzalez-Cortijo L,Murillo R,Sánchez-Bayona R,Cejalvo JM,Gómez-López G,Fustero-Torre C,Sabroso-Lasa S,Malats N,Martinez M,Moreno A,Megias D,Malumbres M,Colomer R,Quintela-Fandino M

PA5-34815 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunohistochemistry (PFA fixed), Immunoprecipitation, Western Blot, Sample Prep to find candidate biomarkers of paclitaxel sensitivity using phosphoproteomic screening of samples from HER2-negative female breast cancer receiving neoadjuvant paclitaxel.

Wed Dec 07 00:00:00 EST 2022

The PP1 regulator PPP1R2 coordinately regulates AURKA and PP1 to control centrosome phosphorylation and maintain central spindle architecture.

BMC molecular and cell biology

Bresch AM,Yerich N,Wang R,Sperry AO

PA5-34815 was used in Immunocytochemistry-immunofluorescence to reveal the involvement of protein phosphatase 1 regulatory subunit 2 in coordinating protein phosphatase 1 and AURKA activity during cytokinesis.

Wed Nov 25 00:00:00 EST 2020

The PP1 regulator PPP1R2 coordinately regulates AURKA and PP1 to control centrosome phosphorylation and maintain central spindle architecture.

BMC molecular and cell biology

Bresch AM,Yerich N,Wang R,Sperry AO

Wed Nov 25 00:00:00 EST 2020

A novel interaction between kinase activities in regulation of cilia formation.

BMC cell biology

DeVaul N,Koloustroubis K,Wang R,Sperry AO

PA5-34815 was used in Immunohistochemistry to demonstrate a complex interplay between Nek2 and AurA kinases in regulation of ciliation in ARPE-19 cells.

Wed Nov 15 00:00:00 EST 2017

生物信息学

蛋白别名: centrosome marker; Gamma-1-tubulin; Gamma-2-tubulin; Gamma-tubulin complex component 1; GCP-1; Tubulin gamma-1 chain; Tubulin gamma-2 chain; tubulin, gamma 1; tubulin, gamma 2; tubulin, gamma polypeptide

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基因别名: 1500010O08Rik; AI451582; AI503389; AI504772; CDCBM4; GCP-1; TUBG; TUBG1; TUBG2; TUBGCP1; Tubgl; zgc:55894

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功能

GTPase activity structural constituent of cytoskeleton protein binding GTP binding structural molecule activity nucleotide binding tubulin

参与通路

G2/M transition of mitotic cell cycle meiotic spindle organization microtubule cytoskeleton organization microtubule nucleation cytoplasmic microtubule organization microtubule-based process mitotic spindle organization regulation of mitotic nuclear division protein polymerization metabolic process mitotic sister chromatid segregation mitotic cell cycle

gamma Tubulin Polyclonal Antibody

gamma Tubulin Antibody (PA5-34815) in ICC/IF

产品信息

PA5-34815

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫组织化学（多聚甲醛固定） (IHC (PFA))

免疫细胞化学 (ICC/IF)

免疫沉淀 (IP)

其他PubMed (Misc)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

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