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iNOS Antibody (MA5-46960) in ICC/IF
Immunofluorescent analysis of iNOS in DIV9 rat cortical neuron and Glia cells. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: iNOS stained by iNOS monoclonal antibody (Product # MA5-46960) diluted at 1:2500. Red: Tau, a Axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI.
产品信息
MA5-46960
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
HL1213
抗原
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse iNOS. The exact sequence is proprietary.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2938032
产品详细信息
Store as concentrated solution.
Centrifuge briefly prior to opening vial.
靶标信息
iNOS (Inducible Nitric oxide, NO, NOS) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. iNOS is expressed in liver and inducible by a combination of lipopolysaccharide and certain cytokines. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Three related iNOS pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. Diseases associated with iNOS dysfunction include achalasia and impotence.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: hepatocytes; inducible nitric oxide synthase; Inducible NO synthase; Inducible NOS; MAC-NOS; Macrophage NOS; nitric oxide synthase 2, inducible, macrophage; Nitric oxide synthase, inducible; nitric oxide synthase-inducible; NOS type II; OTTMUSP00000000202; Peptidyl-cysteine S-nitrosylase NOS2
基因别名: i-NOS; iNOS; Inosl; Nos-2; NOS-II; Nos2; Nos2a
UniProt ID: (Mouse) P29477, (Rat) Q06518
Entrez Gene ID: (Mouse) 18126, (Rat) 24599
actin binding
NADPH-hemoprotein reductase activity
nitric-oxide synthase activity
receptor binding
iron ion binding
protein binding
calmodulin binding
beta-catenin binding
cAMP-dependent protein kinase regulator activity
FMN binding
oxidoreductase activity
protein kinase binding
heme binding
tetrahydrobiopterin binding
arginine binding
protein homodimerization activity
cadherin binding
metal ion binding
flavin adenine dinucleotide binding
nitric-oxide synthase binding
Hsp90 protein binding
amino acid binding
NADP binding
oxidoreductase
protein modifying enzyme
ovulation from ovarian follicle
response to hypoxia
arginine catabolic process
superoxide metabolic process
nitric oxide biosynthetic process
inflammatory response
nitric oxide mediated signal transduction
negative regulation of gene expression
peptidyl-cysteine S-nitrosylation
positive regulation of guanylate cyclase activity
response to lipopolysaccharide
cellular response to drug
regulation of cell proliferation
negative regulation of protein catabolic process
defense response to bacterium
negative regulation of blood pressure
regulation of protein kinase activity
positive regulation of vasodilation
regulation of insulin secretion
positive regulation of killing of cells of other organism
oxidation-reduction process
cellular response to lipopolysaccharide
cellular response to interferon-gamma
endothelial cell proliferation
blood vessel remodeling
signal transduction
G-protein coupled receptor signaling pathway coupled to cGMP nucleotide second messenger
aging
regulation of heart contraction
regulation of blood pressure
response to mechanical stimulus
response to hormone
response to activity
glycoside metabolic process
cGMP-mediated signaling
response to estradiol
response to tumor necrosis factor
intracellular signal transduction
response to immobilization stress
wound healing
positive regulation of apoptotic process
retinoic acid receptor signaling pathway
defense response to Gram-negative bacterium
cellular response to lithium ion
cellular response to vitamin D
cellular response to interleukin-1
cellular response to tumor necrosis factor
cellular response to redox state
response to dexamethasone
