Invitrogen

p38 MAPK beta Monoclonal Antibody (P38-11A5)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

11个已发表图片

18 篇参考文献

查看其他19个p38 MAPK beta抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

p38 MAPK beta Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 18

p38 MAPK beta Antibody (33-8700) in ICC/IF

Immunofluorescent analysis of p38-beta 2 MAPK Antibody (P38-11A5) was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with p38-beta 2 MAPK Antibody (P38-11A5) (Product # 33-8700) at 1µg/mL in 1% BSA and incubated for 3... View More

p38 MAPK beta Antibody in Immunohistochemistry (Paraffin) (IHC (P))

p38 MAPK beta Antibody in Western Blot (WB)

p38 MAPK beta Antibody in Western Blot, Immunoprecipitation (WB, IP)

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产品信息

33-8700

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

查看13篇已发表文章 13篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:10-1:100

免疫细胞化学 (ICC/IF)

1 µg/mL

酶联免疫吸附实验 (ELISA)

Assay-dependent

免疫沉淀 (IP)

Assay-dependent

查看3篇已发表文章 3篇已发表文章

其他PubMed (Misc)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse, Rabbit

宿主/亚型

Mouse / IgG1/IgG2a

分类

Monoclonal

类型

Antibody

克隆号

P38-11A5

抗原

Recombinant, full-length human p38 beta-2; the product of the p38-beta gene

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2533144

产品详细信息

33-8700 has been successfully used in immunofluorescence and western blot.

33-8700 specifically recognizes the beta isoform of p38, with slight reactivity to p38-gamma.

靶标信息

MAPK11 (p38 beta) is a serine/threonine kinase which is involved in a variety of cellular processes, and is activated by proinflammatory cytokines and environmental stress. Mitogen-activated protein kinase 11. The protein encoded by p38 MAPK beta is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. This kinase is most closely related to p38 MAP kinase, both of which can be activated by proinflammatory cytokines and environmental stress. This kinase is activated through its phosphorylation by MAP kinase kinases (MKKs), preferably by MKK6. Transcription factor ATF2/CREB2 has been shown to be a substrate of this kinase.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Crry silencing alleviates Alzheimer's disease injury by regulating neuroinflammatory cytokines and the complement system.

Neural regeneration research

Zhu XC,Liu L,Dai WZ,Ma T

33-8700 was used in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to suggest that Crry silencing in the P301S mouse model reduces tau protein phosphorylation by reducing the levels of neuroinflammation and complement components, thereby improving cognitive function.

Mon Aug 01 00:00:00 EDT 2022

Crry silencing alleviates Alzheimer's disease injury by regulating neuroinflammatory cytokines and the complement system.

Neural regeneration research

Zhu XC,Liu L,Dai WZ,Ma T

Mon Aug 01 00:00:00 EDT 2022

HuR stabilizes HTT mRNA via interacting with its exon 11 in a mutant HTT-dependent manner.

RNA biology

Zhao Q,Li C,Yu M,Sun Y,Wang J,Ma L,Sun X,Lu B

33-8700 was used in Western Blotting to reveal a novel regulatory mechanism of HTT mRNA via non-canonical binding of HuR.

Wed Apr 01 00:00:00 EDT 2020

Discovery of a Small Molecule Promoting Mouse and Human Osteoblast Differentiation via Activation of p38 MAPK-β.

Cell chemical biology

Cook B,Rafiq R,Lee H,Banks KM,El-Debs M,Chiaravalli J,Glickman JF,Das BC,Chen S,Evans T

33-8700 was used in Western Blot to identify activators of the bone marker alkaline phosphatase (ALP), and discover that 6,8-dimethyl-3-(4-phenyl-1H-imidazol-5-yl)quinolin-2(1H)-one (DIPQUO) markedly promotes osteoblast differentiation, including expression of Runx2, Osterix, and Osteocalcin.

Thu Jul 18 00:00:00 EDT 2019

Suppression of MAPK11 or HIPK3 reduces mutant Huntingtin levels in Huntington's disease models.

Cell research

Yu M,Fu Y,Liang Y,Song H,Yao Y,Wu P,Yao Y,Pan Y,Wen X,Ma L,Hexige S,Ding Y,Luo S,Lu B

33-8700 was used in Western Blotting to reveal the kinases MAPK11 and HIPK3 as novel modulators of mutant Huntingtin protein levels, and confirm their therapeutic potentials in Huntington disease knockin mouse models.

Fri Dec 01 00:00:00 EST 2017

Suppression of MAPK11 or HIPK3 reduces mutant Huntingtin levels in Huntington's disease models.

Cell research

Yu M,Fu Y,Liang Y,Song H,Yao Y,Wu P,Yao Y,Pan Y,Wen X,Ma L,Hexige S,Ding Y,Luo S,Lu B

Fri Dec 01 00:00:00 EST 2017

Loss of Functionally Redundant p38 Isoforms in T Cells Enhances Regulatory T Cell Induction.

The Journal of biological chemistry

Hayakawa M,Hayakawa H,Petrova T,Ritprajak P,Sutavani RV,Jiménez-Andrade GY,Sano Y,Choo MK,Seavitt J,Venigalla RKC,Otsu K,Georgopoulos K,Arthur JSC,Park JM

338700 was used in western blot to assess the role of p38 signaling in limiting regulatory T cell induction

Fri Feb 03 00:00:00 EST 2017

Epithelial Control of Gut-Associated Lymphoid Tissue Formation through p38α-Dependent Restraint of NF-κB Signaling.

Journal of immunology (Baltimore, Md. : 1950)

Caballero-Franco C,Guma M,Choo MK,Sano Y,Enzler T,Karin M,Mizoguchi A,Park JM

Published figure using p38 MAPK beta monoclonal antibody (Product # 33-8700) in Western Blot

Tue Mar 01 00:00:00 EST 2016

p38γ and p38δ kinases regulate the Toll-like receptor 4 (TLR4)-induced cytokine production by controlling ERK1/2 protein kinase pathway activation.

Proceedings of the National Academy of Sciences of the United States of America

Risco A,del Fresno C,Mambol A,Alsina-Beauchamp D,MacKenzie KF,Yang HT,Barber DF,Morcelle C,Arthur JS,Ley SC,Ardavin C,Cuenda A

Published figure using p38 MAPK beta monoclonal antibody (Product # 33-8700) in Western Blot

Tue Jul 10 00:00:00 EDT 2012

p38γ and p38δ kinases regulate the Toll-like receptor 4 (TLR4)-induced cytokine production by controlling ERK1/2 protein kinase pathway activation.

Proceedings of the National Academy of Sciences of the United States of America

Risco A,del Fresno C,Mambol A,Alsina-Beauchamp D,MacKenzie KF,Yang HT,Barber DF,Morcelle C,Arthur JS,Ley SC,Ardavin C,Cuenda A

Published figure using p38 MAPK beta monoclonal antibody (Product # 33-8700) in Western Blot

Tue Jul 10 00:00:00 EDT 2012

Cyclosporine A induces nerve growth factor expression via activation of MAPK p38 and NFAT5.

Cornea

Lee JH,Kim JW,Im YS,Seong GJ,Lee HK

33-8700 was used in western blot to study the effects of cyclosporine A on the mechanism of nerve growth factor expression using a corneal epithelial cell line.

Sat Oct 01 00:00:00 EDT 2011

Pomegranate extract inhibits the interleukin-1β-induced activation of MKK-3, p38α-MAPK and transcription factor RUNX-2 in human osteoarthritis chondrocytes.

Arthritis research & therapy

Rasheed Z,Akhtar N,Haqqi TM

33-8700 was used in western blot to study the effect of pomegranate extract on the IL-1beta-induced activation of MKK3/6, p38-MAPK, and RUNX-2 in primary human osteoarthritis chondrocytes

Thu Mar 31 00:00:00 EDT 2011

Pomegranate extract inhibits the interleukin-1β-induced activation of MKK-3, p38α-MAPK and transcription factor RUNX-2 in human osteoarthritis chondrocytes.

Arthritis research & therapy

Rasheed Z,Akhtar N,Haqqi TM

33-8700 was used in western blot to study the effect of pomegranate extract on the IL-1beta-induced activation of MKK3/6, p38-MAPK, and RUNX-2 in primary human osteoarthritis chondrocytes

Thu Mar 31 00:00:00 EDT 2011

Overexpression of mitogen-activated protein kinase kinase 6 in the heart improves functional recovery from ischemia in vitro and protects against myocardial infarction in vivo.

The Journal of biological chemistry

Martindale JJ,Wall JA,Martinez-Longoria DM,Aryal P,Rockman HA,Guo Y,Bolli R,Glembotski CC

33-8700 was used in western blot to use transgenic mice to assess the effects of MKK6-mediated p38 activation in the heart.

Fri Jan 07 00:00:00 EST 2005

Overexpression of mitogen-activated protein kinase kinase 6 in the heart improves functional recovery from ischemia in vitro and protects against myocardial infarction in vivo.

The Journal of biological chemistry

Martindale JJ,Wall JA,Martinez-Longoria DM,Aryal P,Rockman HA,Guo Y,Bolli R,Glembotski CC

33-8700 was used in western blot to use transgenic mice to assess the effects of MKK6-mediated p38 activation in the heart.

Fri Jan 07 00:00:00 EST 2005

BIRB796 inhibits all p38 MAPK isoforms in vitro and in vivo.

The Journal of biological chemistry

Kuma Y,Sabio G,Bain J,Shpiro N,Márquez R,Cuenda A

Fri May 20 00:00:00 EDT 2005

Basic fibroblast growth factor-induced cell death is effected through sustained activation of p38MAPK and up-regulation of the death receptor p75NTR.

The Journal of biological chemistry

Williamson AJ,Dibling BC,Boyne JR,Selby P,Burchill SA

Fri Nov 12 00:00:00 EST 2004

Stress kinase phosphorylation is increased in pacing-induced heart failure in rabbits.

American journal of physiology. Heart and circulatory physiology

Schulz R,Aker S,Belosjorow S,Konietzka I,Rauen U,Heusch G

Sat Nov 01 00:00:00 EST 2003

Stress kinase phosphorylation is increased in pacing-induced heart failure in rabbits.

American journal of physiology. Heart and circulatory physiology

Schulz R,Aker S,Belosjorow S,Konietzka I,Rauen U,Heusch G

Sat Nov 01 00:00:00 EST 2003

Cooperation of p38 and extracellular signal-regulated kinase mitogen-activated protein kinase pathways during granulocyte colony-stimulating factor-induced hemopoietic cell proliferation.

The Journal of biological chemistry

Rausch O,Marshall CJ

Fri Feb 12 00:00:00 EST 1999

生物信息学

蛋白别名: MAP kinase 11; MAP kinase p38 beta; MAPK 11; mitogen activated protein kinase 11; Mitogen-activated protein kinase 11; Mitogen-activated protein kinase p38 beta; mitogen-activated protein kinase p38-2; OTTHUMP00000196655; p38-2; protein kinase, mitogen activated kinase, 11, p38beta; SAPK2b; Stress-activated protein kinase 2b; stress-activated protein kinase-2; stress-activated protein kinase-2b

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基因别名: MAPK11; p38-2; P38B; p38Beta; P38BETA2; PRKM11; SAPK2; SAPK2B

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UniProt ID: (Human) Q15759, (Mouse) Q9WUI1

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Entrez Gene ID: (Human) 5600, (Mouse) 19094

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功能

protein serine/threonine kinase activity MAP kinase activity protein binding ATP binding nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase

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