Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 1
Mouse IgG1 Secondary Antibody (PA1-86329) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of HEL 92.1.7 (Lane 1) and K-562 (Lane 2). The blots were probed with Anti CUL1 Mouse Monoclonal Antibody (Product # 32-2400, 2 µg/mL) and detected by chemiluminescence using Rabbit anti-Mouse IgG1 Secondary Antibody, HRP (Product # PA1-86329) at dilutions 1:2,000 (Fig. 1), 1:10,000 (Fig. 2) and 1:20,000 (Fig. 3). A 89 kDa band corres... View More
产品信息
PA1-86329
应用
建议稀释比
免疫印迹 (WB)
1:2,000-1:10,000
免疫组化（冰冻） (IHC (F))
Assay-dependent
酶联免疫吸附实验 (ELISA)
1:20,000-1:100,000
产品规格
种属反应
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Secondary Antibody
抗原
Mouse IgG, gamma 1 heavy chain.
偶联物
形式
Lyophilized
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS with 1% BSA
内含物
0.01% gentamicin sulfate
保存条件
4° C
运输条件
Wet ice
RRID
AB_933678
靶标
IgG1
抗体形式
Whole Antibody
产品详细信息
PA1-86329 is specific for mouse IgG1 and shows minimal (less than 0.5%) cross-reactivity with mouse IgG2a, IgGb, IgG3 and IgM
Reconstitute with 1.0 mL of distilled water.
Prior to reconstitution store at 4C. After reconstitution store at -20C.
Rabbit anti Mouse IgG1 antibody is specific for mouse IgG1 and shows minimal (<0.5%) cross-reactivity with mouse IgG2a, IgG2b, IgG3 and IgM.
靶标信息
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
