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产品规格
检测灵敏度
1.85 pg/mL
测定范围
8.2-2000 pg/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL, Urine=50 µL, Fecal Extract=50 µL}
手工操作时间
1 hr 20 min
总时间
2 hr 30 min
均相（无需洗涤）
No
批间差异
<8%
批内差异
<6%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, Antibody, HRP-Conjugated Detection Antibody, Assay Buffer Concentrate, Wash Buffer, TMB Substrate, Stop Solution, Adhesive Plate Covers
运输条件
Wet ice
储存
-20°C
蛋白名称
11-Ketotestosterone
蛋白家族
Chromone
均相（无需洗涤）
Chemical
检测试剂盒格式
Competitive ELISA
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The multispecies 11-Ketotestosterone ELISA quantitates 11-Ketotestosterone in extracted serum, plasma, and dried fecal samples, or urine.
原理方法:
The 11-Ketotestosterone Competitive ELISA research-use-only kit is designed to quantitatively measure 11-Ketotestosterone independent of species. A 11-Ketotestosterone standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with goat anti-rabbit IgG antibodies. 11-Ketotestosterone polyclonal rabbit antibodies and 11-Ketotestosterone-peroxidase conjugate is added to the wells. The 11-Ketotestosterone conjugate and any 11-Ketotestosterone in the sample will compete to bind to the 11-Ketotestosterone polyclonal rabbit antibodies. After incubation, the plate is washed and substrate is added. The substrate reacts with the bound 11-Ketotestosteron-peroxidase conjugate. After a 30 min incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm. The intensity of the generated color corresponds inversely to the amount of 11-Ketotestosterone present in the sample.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Androgenic hormones, such as testosterone, affect many male organisms’ growth, size, and reproduction. In teleost fish, along with testosterone, 11-ketotestosterone plays a significant role by inducing reproductive characteristics in both males and females. In particular male fish, 11-ketotestosterone levels increase during spermatogenesis in the spawning season, while in some female fish, 11-ketotestosterone increases before yolk deposition to regulate ovarian development.
The presence and involvement of 11-ketotestosterone in other species, such as humans, have only recently been established. In contrast to fish, primate serum 11-ketotestosterone concentrations were not significantly different in males and females, despite males having significantly higher circulating testosterone. This evidence suggests that 11-ketotestosterone production in these species may not be testis-dependent and primarily originates from adrenal-derived 11-oxyandrogen precursors. Recent studies have discovered more 11-ketotestosterone than its precursors, androstenedione, and testosterone, in prepubertal children and postmenopausal women compared to men. This discovery highlights the potential use of 11-ketotestosterone as a clinical biomarker to screen adrenal androgen excess in disease conditions such as polycystic ovary syndrome (PCOS) and hirsutism in women.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名 : 17-Hydroxyandrost-4-ene-3, 11-dione, 11-Ketotestosterone, 11 Oxotestosterone, 11KT, 11-KT
