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产品规格
检测灵敏度
<15.6 pg/mL
测定范围
15.6-1000 pg/mL
检测样本类型/体积
{Serum=100 µL, Supernatant=100 µL}
手工操作时间
24 hr
总时间
3 hr 20 min
均相（无需洗涤）
No
检测仪器
Colorimetric Microplate Reader
产品规格
5 x 96 Tests
产品成分
Coating Antibody
Lyophilized Recombinant Standard
Detection Antibody
Streptavidin-HRP
Substrate Solution
Ready to use Stop Solution
0.16M Sulfuric Acid
Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
Lyophilized Recombinant Standard
Detection Antibody
Streptavidin-HRP
Substrate Solution
Ready to use Stop Solution
0.16M Sulfuric Acid
Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
运输条件
Wet ice
储存
2-8°C
蛋白名称
IL-4
均相（无需洗涤）
Bovine
检测试剂盒格式
Uncoated ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Bovine IL-4 Uncoated ELISA Kit contains pre-matched antibody pairs and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of bovine IL-4.
原理方法
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
靶标信息
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : BSF-1, IL-4, IL4
基因ID : (Bovine) 280824
基因符号 : IL4
蛋白别名 : B-cell stimulatory factor 1, BSF-1, IL-4, Interleukin-4, Lymphocyte stimulatory factor 1
UniProt ID (Bovine) P30367
