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产品规格
检测灵敏度
<15.6 pg/mL
测定范围
15.6-1000 pg/mL
检测样本类型/体积
{Plasma=100 µL, Serum=100 µL, Supernatant=100 µL}
手工操作时间
1 hr 30 min
总时间
24 hr
均相（无需洗涤）
No
检测仪器
Colorimetric Microplate Reader
产品规格
10 x 96 Tests
产品成分
Capture (coating) Antibody
Detection Antibody
Standard
Streptavidin-HRP
Detection Antibody
Standard
Streptavidin-HRP
运输条件
Wet ice (domestic); Wet or Dry ice (international)
储存
2-8°C
蛋白名称
IFN gamma
蛋白亚型
Adaptor Proteins
均相（无需洗涤）
Chicken
检测试剂盒格式
Matched Antibody Pair
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Chicken Interferon gamma Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of chicken IFNγ. Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No. CNB0011). The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate.
原理方法
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
靶标信息
IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : IFNG
基因ID : (Chicken) 396054
基因符号 : IFNG
蛋白别名 : IFN-gamma, Interferon gamma, interferon-gama, Interferon-gamma level
UniProt ID (Chicken) P49708
