Search Thermo Fisher Scientific
![Human AMPK alpha-1,2 (Phospho) [pT172] ELISA Kit](https://www.thermofisher.cn/antibody/images/650/KHO0651_SC_080326.jpg?time=20220826 "Human AMPK alpha-1,2 (Phospho) [pT172] ELISA Kit")
![Human AMPK alpha-1,2 (Phospho) [pT172] ELISA Kit](https://www.thermofisher.cn/antibody/images/150/KHO0651_SC_080326.jpg?time=20220826)
![Human AMPK alpha-1,2 (Phospho) [pT172] ELISA Kit (KHO0651)](https://www.thermofisher.cn/antibody/images/150/Sandwich-Direct-ELISA-Kit-Packaging-650x600.jpg?time=20220826)
产品规格
检测灵敏度
<1 U/mL
测定范围
< 1 U/mL
检测样本类型/体积
{Cell Lysate=10 µL}
手工操作时间
1 hr 20 min
总时间
4 hr
均相（无需洗涤）
No
批间差异
7.6%
批内差异
4.8%
检测仪器
Colorimetric Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Standard Dilution Buffer
Detection Antibody
anti-rabbit-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Standard Dilution Buffer
Detection Antibody
anti-rabbit-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
AMPK alpha-1,2
Target site-specificity
Phosphorylated Thr172
蛋白亚型
Serine/Threonine Kinases
蛋白家族
Kinases & Inhibitors
均相（无需洗涤）
Human, Mouse, Rat
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Human AMPK alpha-1,2 (Phospho) [pT172] ELISA Kit is a solid-phase sandwich Enzyme-Linked Immunosorbent Assay (ELISA) designed to detect and quantify the level of AMPK alpha-1,2 (Phospho) [pT172] in fresh or frozen human cell lysates. Cross-reactivity has been observed in mouse and rat cells. The assay recognizes both natural and recombinant human AMPK alpha-1,2 (Phospho) [pT172].
原理方法
A monoclonal capture antibody specific for AMPK alpha-1,2 (Phospho) [pT172] has been coated onto the wells of the 96-well plate. During the first incubation, standards of known content and unknown samples are pipetted into the wells and the antigen binds to the immobilized (capture) antibody. After washing, a rabbit antibody specific for the target protein is added to the wells and serves as a detection antibody by binding to the immobilized protein captured during the first incubation. After washing, a horseradish peroxidase labeled anti-rabbit IgG is added. This binds to the detection antibody to complete the four member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of target protein present in the original specimen and the optical density can be read on a standard microplate reader.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : 2310008I11Rik, A830082D05, AI194361, AI450832, AL024255, AMPK, AMPK1, AMPK2, AMPKa1, AMPKa2, AMPKalpha1, AMPKalpha2, C130083N04Rik, PRKAA, PRKAA1, PRKAA2
基因符号 : PRKAA1, PRKAA2, Prkaa1, Prkaa1, Prkaa2, Prkaa2
蛋白别名 : 5'-AMP-activated protein kinase catalytic subunit alpha-1, 5'-AMP-activated protein kinase catalytic subunit alpha-2, 5'-AMP-activated protein kinase, catalytic alpha-1 chain, catalytic alpha-2 chain, ACACA kinase, Acetyl-CoA carboxylase kinase, AMP -activate kinase alpha 1 subunit, AMP-activated protein kinase, AMP-activated protein kinase alpha-2 subunit variant 2, AMP-activated protein kinase alpha-2 subunit variant 3, AMP-activated protein kinase alpha-2 variant B, alpha 1 catalytic subunit, catalytic, alpha-1, AMPK alpha 1, AMPK alpha 2, AMPK alpha-1 chain, AMPK alpha-2 chain, AMPK subunit alpha-1, AMPK subunit alpha-2, AMPK-alpha-2 chain, HMGCR kinase, Hydroxymethylglutaryl-CoA reductase kinase, prkaa2, protein kinase, AMP-activated, alpha 2 catalytic subunit, Tau-protein kinase PRKAA1
UniProt ID (Human) Q13131, (Human) P54646, (Rat) P54645, (Mouse) Q5EG47, (Rat) Q09137, (Mouse) Q8BRK8
