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产品规格
检测灵敏度
2.60 pg/mL
测定范围
15.6-1,000 pg/mL
检测样本类型/体积
{Plasma=10 µL, Serum=10 µL, Supernatant=10 µL}
手工操作时间
15 min
总时间
3 hr 30 min
均相（无需洗涤）
No
批间差异
3.3%
批内差异
4.6%
检测仪器
Colorimetric Microplate Reader
产品规格
128 Tests
产品成分
Pre-coated 96 well Microplate
Biotinylated Detection Antibody
SAV-HRP
Sample Diluent, lyophilized
Standard (8-well strips x 4)
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Lysis Buffer 10x
Adhesive Plate Covers
Biotinylated Detection Antibody
SAV-HRP
Sample Diluent, lyophilized
Standard (8-well strips x 4)
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Lysis Buffer 10x
Adhesive Plate Covers
运输条件
Wet ice
储存
-20°C
蛋白名称
CD105 (Endoglin)
Target sub-specificity
Soluble
均相（无需洗涤）
Human
检测试剂盒格式
Instant ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human Soluble CD105/Endoglin (Hu sCD105) ELISA quantitates Hu sCD105 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu sCD105.
原理方法
The Human sCD105 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists on an O- and N-glycosylated homodimer. Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker. CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : END, ENG, HHT1, ORW1
基因ID : (Human) 2022
基因符号 : ENG
蛋白别名 : CD105, CD105 antigen, Endoglin
UniProt ID (Human) P17813
