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The Human Corticotropin Releasing Hormone (CRH) ELISA quantitates CRH in serum, plasma and other biological fluids
原理方法:
The Competitive Corticotropin Releasing Hormone (CRH) ELISA research-use-only kit is designed to quantitatively measure CRH in Humans. A CRH standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to Human CRH is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of Human CRH in the samples is then determined by comparing the OD of the samples to the standard curve.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Corticotropin Releasing Factor (CRF), a 41 amino acids neuropeptide, belongs to a family of structurally related peptides including urocortin, sauvagine and urotensin I. CRF is critical in the regulation of the pituitary-adrenal gland axis, and in endocrine, autonomic and behavioral responses to stress. CRF is widely distributed in the brain and peripheral nervous system with the highest levels in the hypothalamic paraventricular nucleus.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
基因别名 : CRF, CRH, CRH1
基因ID : (Human) 1392
基因符号 : CRH
蛋白别名 : Corticoliberin, Corticotropin-releasing factor, Corticotropin-releasing hormone, CRF
UniProt ID (Human) P06850