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产品规格
检测灵敏度
0.14 ng/mL
测定范围
0.3-20 ng/mL
检测样本类型/体积
{Plasma=10 µL, Serum=10 µL, Supernatant=50 µL}
手工操作时间
1 hr 20 min
总时间
4 hr 30 min
均相（无需洗涤）
No
批间差异
5.9%
批内差异
5.9%
检测仪器
Colorimetric Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
Complement C3a
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human Complement C3a (Hu C3a) ELISA quantitates Hu C3a in human serum, plasma or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu C3a.
原理方法
The Human C3a solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. People with C3 deficiency are susceptible to bacterial infection.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : AHUS5, ARMD9, ASP, C3, C3a, C3b, CPAMD1, HEL-S-62p
基因ID : (Human) 718
基因符号 : C3
蛋白别名 : acylation-stimulating protein cleavage product, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, C3a anaphylatoxin, Complement C3, complement component C3a, complement component C3b, epididymis secretory sperm binding protein Li 62p, prepro-C3
UniProt ID (Human) P01024
