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The Human DIABLO (Hu SMAC) ELISA quantitates Hu SMAC in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu SMAC.
原理方法
The Human SMAC solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
DIABLO (smac), a mitochondrial protein, activates various forms of apoptosis. This activation may be due to the neutralization of one or more members of the IAP family of apoptosis inhibitory proteins. Smac exits the mitochondria and enter the cytosol during certain apoptosis triggered events. Mitochondrial-mediated apoptosis is important in animal development and tissue homeostasis, with alterations resulting in a range of malignant disorders. Upon apoptotic stimuli, the mitochondrial proteins cytochrome c and Smac/DIABLO are released into the cytosol. The release of these proteins, however, occurs via different mechanisms. Smac/DIABLO has also been found to play a key role in regulating the sensitization of cancer cells to apoptosis (both immune and drug-induced).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
基因别名 : DFNA64, DIABLO, SMAC
基因ID : (Human) 56616
基因符号 : DIABLO
蛋白别名 : Diablo homolog, mitochondrial, Diablo IAP-binding mitochondrial protein, diablo, IAP-binding mitochondrial protein, diablo-like protein, Direct IAP-binding protein with low pI, Second mitochondria-derived activator of caspase, Smac
UniProt ID (Human) Q9NR28