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The Human Soluble Endothelial Leukocyte Adhesion Molecule-1 (ELAM-1) (Hu E-selectin) ELISA quantitates Hu sE-Selectin in human serum, plasma, cell culture supernatants, urine, amniotic fluid, bile, or other body fluids. The assay will exclusively recognize both natural and recombinant Hu sE-Selectin
原理方法
The Human sE-Selectin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkB dependent signaling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
基因别名 : CD62E, ELAM, ELAM1, ESEL, LECAM2, SELE
基因ID : (Human) 6401
基因符号 : SELE
蛋白别名 : CD62 antigen-like family member E, CD62E, E-selectin, ELAM-1, endothelial adhesion molecule 1, Endothelial leukocyte adhesion molecule 1, LECAM2, leukocyte endothelial cell adhesion molecule 2, Leukocyte-endothelial cell adhesion molecule 2
UniProt ID (Human) P16581