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![Human EGFR (Phospho) [pY1173] ELISA Kit](https://www.thermofisher.cn/antibody/images/650/KHR9071_SC_080327.jpg?time=20220826 "Human EGFR (Phospho) [pY1173] ELISA Kit")
![Human EGFR (Phospho) [pY1173] ELISA Kit](https://www.thermofisher.cn/antibody/images/150/KHR9071_SC_080327.jpg?time=20220826)
![Human EGFR (Phospho) [pY1173] ELISA Kit](https://www.thermofisher.cn/antibody/images/150/4115.jpg?time=20220826)
![Human EGFR (Phospho) [pY1173] ELISA Kit (KHR9071)](https://www.thermofisher.cn/antibody/images/150/Sandwich-Direct-ELISA-Kit-Packaging-650x600.jpg?time=20220826)
产品规格
检测灵敏度
<0.3 U/mL
测定范围
1.6-100 U/mL
检测样本类型/体积
{Cell Lysate=10 µL}
手工操作时间
1 hr 20 min
总时间
4 hr
均相（无需洗涤）
No
批间差异
6.6%
批内差异
4.9%
检测仪器
Colorimetric Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Standard Dilution Buffer
Detection Antibody
anti-rabbit-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Standard Dilution Buffer
Detection Antibody
anti-rabbit-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
EGFR
Target site-specificity
Phosphorylated Tyr1173
蛋白亚型
Receptor Tyrosine Kinases
蛋白家族
Growth Factors & Receptors, Kinases & Inhibitors
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Human EGFR (Phospho) [pY1173] ELISA Kit is a solid-phase sandwich Enzyme-Linked Immunosorbent Assay (ELISA) designed to detect and quantify the level of EGFR (Phospho) [pY1173]) in fresh or frozen human cell lysates. The assay recognizes both natural and recombinant human EGFR(Phospho) [pY1173].
原理方法
A monoclonal capture antibody specific for EGFR (Phospho) [pY1173]) has been coated onto the wells of the 96-well plate. During the first incubation, standards of known content and unknown samples are pipetted into the wells and the antigen binds to the immobilized (capture) antibody. After washing, a rabbit antibody specific for the target protein is added to the wells and serves as a detection antibody by binding to the immobilized protein captured during the first incubation. After washing, a horseradish peroxidase labeled anti-rabbit IgG is added. This binds to the detection antibody to complete the four member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of target protein present in the original specimen and the optical density can be read on a standard microplate reader.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
EGFR, epidermal growth factor receptor, is a receptor tyrosine kinases that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics. EGFR is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : EGFR, ERBB, ERBB1, HER1, mENA, NISBD2, PIG61
基因ID : (Human) 1956
基因符号 : EGFR
蛋白别名 : avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, Epidermal growth factor receptor, erb-b2 receptor tyrosine kinase 1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
UniProt ID (Human) P00533
