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产品规格
检测灵敏度
30 pg/mL
测定范围
30-4,000 pg/mL
检测样本类型/体积
{Plasma=100 µL, Serum=100 µL, Supernatant=100 µL}
手工操作时间
1 hr 45 min
总时间
24 hr 30 min
均相（无需洗涤）
No
检测仪器
Colorimetric Microplate Reader
产品规格
10 x 96 Tests
产品成分
Capture Antibody: Pre-titrated, purified antibody
Detection Antibody: Pre-titrated, biotin-conjugated antibody
Standard: Recombinant cytokine for generating standard curve and calibrating samples
Assay Diluent: 5X concentrated
Wash Buffer: 10X concentrated (100 mL of 10X per plate)
Detection enzyme: Pre-titrated Avidin-HRP
Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution
Stop Solution: 5 mLs of 1X solution per plate
Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
Detection Antibody: Pre-titrated, biotin-conjugated antibody
Standard: Recombinant cytokine for generating standard curve and calibrating samples
Assay Diluent: 5X concentrated
Wash Buffer: 10X concentrated (100 mL of 10X per plate)
Detection enzyme: Pre-titrated Avidin-HRP
Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution
Stop Solution: 5 mLs of 1X solution per plate
Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
运输条件
Wet ice
储存
2-8°C
蛋白名称
IL-17AF
均相（无需洗涤）
Human
检测试剂盒格式
Uncoated ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human IL-17AF (heterodimer) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-17AF. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.
原理方法
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
靶标信息
IL-17A and IL-17F are well-characterized homodimeric cytokines secreted by T helper 17 (Th17) cells, gamma-delta T cells and several subsets of innate lymphoid cells. Somewhat less appreciated, IL-17A and IL-17F subunits can also form the heterodimer, IL-17AF. Together, these three dimers signal through the IL-17RA/IL-17RC receptor complex to mediate immune responses at mucosal interfaces and are found at lesion sites in inflammatory bowel disease, asthma, atopic dermatitis and rheumatoid arthritis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : CANDF6, CTLA-8, CTLA8, IL-17, IL-17A, IL-17F, IL17, IL17A, IL17F, ML-1, ML1
基因ID : (Human) 3605, (Human) 112744
基因符号 : IL17A, IL17F
蛋白别名 : CTLA-8, Cytokine ML-1, Cytotoxic T-lymphocyte-associated antigen 8, cytotoxic T-lymphocyte-associated protein 8, IL-17, IL-17A, IL-17F, interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8), Interleukin-17A, Interleukin-17F
UniProt ID (Human) Q16552, (Human) Q96PD4
