Search Thermo Fisher Scientific
产品规格
检测灵敏度
0.2 pg/mL
测定范围
0.94-60.0 pg/ml
检测样本类型/体积
{Plasma=50 µL, Supernatant=50 µL, Serum=50 µL}
手工操作时间
1 hr 20 min
总时间
4 hr
均相（无需洗涤）
No
批间差异
6.2%
批内差异
5.7%
检测仪器
Colorimetric Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated microwell plate
Biotinylated Detection Antibody
Streptavidin-HRP
Standard
Control (High)
Control (Low)
Sample Diluent
Assay Buffer Concentrate
Amplification Reagent I
Amplification Reagent II
Wash Buffer Concentrate
Substrate Solution
Stop Solution
Adhesive Plate Covers
Biotinylated Detection Antibody
Streptavidin-HRP
Standard
Control (High)
Control (Low)
Sample Diluent
Assay Buffer Concentrate
Amplification Reagent I
Amplification Reagent II
Wash Buffer Concentrate
Substrate Solution
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
IL-2
均相（无需洗涤）
Human
检测试剂盒格式
High Sensitivity Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human High Sensitivity Interleukin 2 (HS IL-2) ELISA quantitates Hu HS IL-2 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu HS IL-2.
原理方法
The Human HS IL-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A special amplification reagent step is added to achieve the high sensitivity capability. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
基因别名 : IL-2, IL2, lymphokine, TCGF
基因ID : (Human) 3558
基因符号 : IL2
蛋白别名 : aldesleukin, Aldesleukin, IL-2, T-cell growth factor, TCGF, Interleukin-2, involved in regulation of T-cell clonal expansion, T cell growth factor
UniProt ID (Human) P60568
